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首页> 外文期刊>Biology Open >Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in?Drosophila
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Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in?Drosophila

机译：果蝇血脑屏障形成胶质细胞间质-上皮转化的动态分析

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During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in?Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments.?In vivo?time-lapse imaging reveals that the Moody GPCR signaling pathway regulates SPG cell growth and shape, with different levels of signaling causing distinct phenotypes. Timely, well-coordinated SPG growth is essential for the uniform insertion of SJs and thus the insulating function of the barrier. To our knowledge, this is the first dynamic?in vivo?analysis of all stages in the formation of a secondary epithelium, and of the key role trimeric G protein signaling plays in this important morphogenetic process.
机译：在发育过程中，许多上皮细胞是由间充质-上皮转化（MET）形成的。在这里，我们检查果蝇血脑屏障形成过程中MET的主要阶段和潜在机制。我们显示与基底层的接触对于形成屏障的会阴部胶质下神经胶质细胞（SPG）的生长至关重要。分隔连接（SJs）可提供细胞旁空间的绝缘，但MET并不需要，但对于建立极化SPG膜区室而言是必需的。体内时移成像显示，穆迪GPCR信号通路调节SPG细胞生长和形状，不同水平的信号传导导致不同的表型。及时，协调良好的SPG增长对于SJ的均匀插入以及屏障的绝缘功能至关重要。据我们所知，这是继发上皮形成过程中所有阶段的首次动态体内分析，也是三聚体G蛋白信号在这一重要的形态发生过程中所起的关键作用。

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