Measurement of Rabbit Apolipoprotein B by Use of Electroimmunodiffusion and Immunonephelometric Assays

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Measurement of Rabbit Apolipoprotein B by Use of Electroimmunodiffusion and Immunonephelometric Assays

机译：电免疫扩散和免疫比色法测定兔载脂蛋白B

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BackgroundandPurpose:Becauseoftheirsimilaritytohumans,rabbitsareagoodanimalmodelforthestudyofatherosclerosisassociatedwithhighserumlow-densitylipoprotein(LDL)values.TwoassaysweredevelopedtomeasureapolipoproteinB(apoB),themajorstructuralandfunctionalapolipoproteinofLDLinrabbitsandtodistinguishendogenousLDLintransgenicrabbitsfromthatofhumanapoB.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:Twoprocedures,anelectroimmunoassay(EIA)andanimmunonephelometricassay(INA),alongwithagoat-originrabbitantiserumweredevelopedtomeasureserumapoBconcentrationinrabbits.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults:Useofeitherassayresultedinabilitytomeasurerabbitspecies-specificapoBconcentration.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusion:Theseassaysshouldhavebroadapplications:toscreencompoundsordietsthatmightlowerserumapoBconcentrations;tospecificallymeasurehumanapoBconcentrationintransgenicrabbits;tomeasureserumapoBconcentrationinrabbitsoverexpressingotherhumanproteins.

机译：BackgroundandPurpose：Becauseoftheirsimilaritytohumans，rabbitsareagoodanimalmodelforthestudyofatherosclerosisassociatedwithhighserumlow-densitylipoprotein（LDL）values.TwoassaysweredevelopedtomeasureapolipoproteinB（载脂蛋白B），themajorstructuralandfunctionalapolipoproteinofLDLinrabbitsandtodistinguishendogenousLDLintransgenicrabbitsfromthatofhumanapoB 方法：Twoprocedures，anelectroimmunoassay（EIA ）和免疫比尔测定法（INA），以及用于起源的兔血清的研究都已开发出来。结果：使用两种测定结果都可用于测量兔子物种的比例。结论：Theseassays应该有广泛的应用程序：筛选可能降低erumapoB浓度的化合物或饮食;特定地测量转基因兔中人类apoB的浓度;来测量serumapoB浓度在兔子身上的过量表达摄取其他人类蛋白质。

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《Comparative Medicine》 |2000年第3期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Measurement of Rabbit Apolipoprotein B by Use of Electroimmunodiffusion and Immunonephelometric Assays [J] . Anne Tailleux, Julien Labbe, Celine Mallet, Comparative Medicine . 2000,第3期

机译：电免疫扩散和免疫比色法测定兔载脂蛋白B
2. Electroimmuno- and immunonephelometric assays of apolipoprotein A-I by using a mixture of monoclonal antibodies. [J] . C Durieux, C Fievet, I Pruvot, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 1988,第10期

机译：通过使用单克隆抗体的混合物进行载脂蛋白A-1的电免疫和免疫比浊法测定。
3. Immunonephelometric assay of human apolipoprotein AI. [J] . G Evans, G Virella, J A Colwell, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 1980,第8期

机译：人类载脂蛋白AI的免疫电泳测定。
4. Total Apolipoprotein E and Apolipoprotein E4 isoform assay (n=672) by targeted mass spectrometry: a pilot assay for methionine-containing proteotypic peptide [C] . Romain Simon, Marion Girod, Catherine Fonbonne, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：通过靶向质谱法（N = 672）总载脂蛋白E和载脂蛋白E4同种型测定：含甲硫氨酸的蛋白质肽的试验试验
5. Development of a Microfluidic Viscoelastic Hemostatic Assay for Real-Time Viscosity Measurements of Blood [D] . Kent, Shay. 2021

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6. The extended lipid panel assay: a clinically-deployed high-throughput nuclear magnetic resonance method for the simultaneous measurement of lipids and Apolipoprotein B [O] . Erwin Garcia, Dennis W. Bennett, Margery A. Connelly, 2020

机译：延长的脂基板测定：临床部署的高通量核磁共振方法用于同时测量脂质和载脂蛋白B.
7. The extended lipid panel assay: a clinically-deployed high-throughput nuclear magnetic resonance method for the simultaneous measurement of lipids and Apolipoprotein B [O] . Erwin Garcia, Dennis W. Bennett, Margery A. Connelly, 2020

机译：延长的脂基板测定：临床部署的高通量核磁共振方法，用于同时测量脂质和载脂蛋白B.
8. Allyl Alcohol Dose Range Finding Developmental Screening Assay with New Zealand White Rabbits. [R] . 2012

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Measurement of Rabbit Apolipoprotein B by Use of Electroimmunodiffusion and Immunonephelometric Assays

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