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首页> 外文期刊>Biology Open >Analysis of neural progenitors from embryogenesis to juvenile adult in Xenopus laevis reveals biphasic neurogenesis and continuous lengthening of the cell cycle
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Analysis of neural progenitors from embryogenesis to juvenile adult in Xenopus laevis reveals biphasic neurogenesis and continuous lengthening of the cell cycle

机译：对非洲爪蟾从胚胎发生到成年成年的神经祖细胞的分析揭示了双相神经发生和细胞周期的持续延长

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Xenopus laevis is a prominent model system for studying neural development, but our understanding of the long-term temporal dynamics of neurogenesis remains incomplete. Here, we present the first continuous description of neurogenesis in X. laevis , covering the entire period of development from the specification of neural ectoderm during gastrulation to juvenile frog. We have used molecular markers to identify progenitors and neurons, short-term bromodeoxyuridine (BrdU) incorporation to map the generation of newborn neurons and dual pulse S-phase labelling to characterise changes in their cell cycle length. Our study revealed the persistence of Sox3-positive progenitor cells from the earliest stages of neural development through to the juvenile adult. Two periods of intense neuronal generation were observed, confirming the existence of primary and secondary waves of neurogenesis, punctuated by a period of quiescence before metamorphosis and culminating in another period of quiescence in the young adult. Analysis of multiple parameters indicates that neural progenitors alternate between global phases of differentiation and amplification and that, regardless of their behaviour, their cell cycle lengthens monotonically during development, at least at the population level.
机译：非洲爪蟾是研究神经发育的重要模型系统，但是我们对神经发生的长期时间动态的理解仍然不完整。在这里，我们提出了X. laevis中神经发生的第一个连续描述，涵盖了从胃胚形成到幼蛙的神经外胚层规范的整个发展时期。我们已经使用分子标记物鉴定祖细胞和神经元，并结合了短期溴脱氧尿苷（BrdU）来绘制新生神经元的图谱，并使用了双脉冲S期标记来表征其细胞周期长度的变化。我们的研究揭示了Sox3阳性祖细胞从神经发育的最早阶段一直持续到少年。观察到两个阶段的强烈神经元生成，证实了神经发生的初级和次级波的存在，在变态之前由一段静止期中断，并在年轻成年人中达到另一个静止期。多个参数的分析表明，神经祖细胞在分化和扩增的总体阶段之间交替，并且不管其行为如何，它们的细胞周期在发育过程中至少在群体水平上单调延长。

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