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首页> 外文期刊>Biology Open >The circular RNA PVT1/miR-203/HOXD3 pathway promotes the progression of human hepatocellular carcinoma
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The circular RNA PVT1/miR-203/HOXD3 pathway promotes the progression of human hepatocellular carcinoma

机译：环状RNA PVT1 / miR-203 / HOXD3途径促进人类肝细胞癌的发展

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Accumulating evidence suggests that circular RNAs (circRNAs) play important roles in various physiological and pathological processes. In the present study, we explored the role of circRNA PVT1 in hepatocellular carcinoma (HCC). qRT-PCR was performed to detect the relative expression of circPVT1 in HCC tissues and cell lines. The oncogenic roles of circPVT1 in HCC were evaluated by cell counting kit-8 (CCK-8) assay, ethynyl deoxyuridine (EdU) incorporation assays, transwell assays, flow cytometry andin vivoxenograft growth. Furthermore, bioinformatics, luciferase reporter assays and rescue experiments were conducted to determine the underlying mechanism of circPVT1 in HCC. Enhanced circPVT1 expression was detected in HCC tissues, which was closely associated with poor prognosis of patients with HCC. Knockdown of circPVT1 decreased the proliferation and migration ability of HCC cell linesin vitro. Conversely, upregulation of circPVT1 improved the growth and migration in HCC cells. Mechanistically, we found that circPVT1 could bind directly to miR-203 and contributed to the initiation and progression of HCC by regulating miR-203/homebox D3 (HOXD3) pathway. In conclusion, our study reveals that circPVT1 participates in the progression of HCC through the miR-203/homeobox D3 (HOXD3) pathway and might represent a potential therapeutic target for HCC treatment.
机译：越来越多的证据表明，环状RNA（circRNA）在各种生理和病理过程中起着重要作用。在本研究中，我们探讨了circRNA PVT1在肝细胞癌（HCC）中的作用。进行qRT-PCR检测circPVT1在HCC组织和细胞系中的相对表达。通过细胞计数试剂盒8（CCK-8）分析，乙炔基脱氧尿苷（EdU）掺入分析，transwell分析，流式细胞术和体内异种生长评估了circPVT1在HCC中的致癌作用。此外，进行了生物信息学，荧光素酶报告基因测定和拯救实验，以确定circPVT1在肝癌中的潜在机制。在肝癌组织中检测到增强的circPVT1表达，这与肝癌患者的不良预后密切相关。敲除circPVT1可以降低HCC细胞系的体外增殖和迁移能力。相反，circPVT1的上调改善了HCC细胞的生长和迁移。从机理上讲，我们发现circPVT1可以直接与miR-203结合，并通过调节miR-203 / homebox D3（HOXD3）途径促进HCC的发生和发展。总之，我们的研究表明circPVT1通过miR-203 / homeobox D3（HOXD3）途径参与了HCC的进程，可能代表了HCC治疗的潜在治疗靶点。

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