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首页> 外文期刊>Biology Open >Msx1 is expressed in retina endothelial cells at artery branching sites
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Msx1 is expressed in retina endothelial cells at artery branching sites

机译：Msx1在动脉分支部位的视网膜内皮细胞中表达

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Msx1 and Msx2 encode homeodomain transcription factors that play a role in several embryonic developmental processes. Previously, we have shown that in the adult mouse, Msx1lacZ is expressed in vascular smooth muscle cells (VSMCs) and pericytes, and that Msx2lacZ is also expressed in VSMCs as well as in a few endothelial cells (ECs). The mouse retina and choroid are two highly vascularized tissues. Vessel alterations in the retina are associated with several human diseases and the retina has been intensely used for angiogenesis studies, whereas the choroid has been much less investigated. Using the Msx1lacZ and Msx2lacZ reporter alleles, we observed that Msx2 is not expressed in the eye vascular tree in contrast to Msx1 , for which we establish the spatial and temporal expression pattern in these tissues. In the retina, expression of Msx1 takes place from P3, and by P10, it becomes confined to a subpopulation of ECs at branching points of superficial arterioles. These branching sites are characterized by a subpopulation of mural cells that also show specific expression programs. Specific Msx gene inactivation in the endothelium, using Msx1 and Msx2 conditional mutant alleles together with a Tie2-Cre transgene, did not lead to conspicuous structural defects in the retinal vascular network. Expression of Msx1 at branching sites might therefore be linked to vessel physiology. The retinal blood flow is autonomously regulated and perfusion of capillaries has been proposed to depend on arteriolar precapillary structures that might be the sites for Msx1 expression. On the other hand, branching sites are subject to shear stress that might induce Msx1 expression. In the choroid vascular layer Msx1lacZ is expressed more broadly and dynamically. At birth Msx1lacZ expression takes place in the endothelium but at P21 its expression has shifted towards the mural layer. We discuss the possible functions of Msx1 in the eye vasculature.
机译：Msx1和Msx2编码在几个胚胎发育过程中起作用的同源域转录因子。以前，我们已经证明，在成年小鼠中，Msx1lacZ在血管平滑肌细胞（VSMC）和周细胞中表达，并且Msx2lacZ在VSMC和一些内皮细胞（EC）中也表达。小鼠视网膜和脉络膜是两个高度血管化的组织。视网膜中的血管改变与几种人类疾病有关，视网膜已被广泛用于血管生成研究，而脉络膜的研究较少。使用Msx1lacZ和Msx2lacZ报告基因，我们观察到与Msx1相比，Msx2在眼血管树中不表达，为此我们在这些组织中建立了时空表达模式。在视网膜中，Msx1的表达从P3发生，到P10时，它局限于表皮小动脉分支点的ECs亚群。这些分支位点的特征是壁细胞的亚群，也显示特定的表达程序。内皮中的特定Msx基因失活，使用Msx1和Msx2条件突变等位基因与Tie2-Cre转基因一起，不会导致视网膜血管网络中明显的结构缺陷。因此，Msx1在分支部位的表达可能与血管生理有关。视网膜血流是自主调节的，并且毛细血管的灌注已被提出取决于小动脉毛细血管前结构，该结构可能是Msx1表达的位点。另一方面，分支部位受到剪切应力的影响，这可能会诱导Msx1表达。在脉络膜血管层中，Msx1lacZ更广泛，更动态地表达。在出生时，Msx1lacZ表达在内皮中发生，但在P21时，其表达已向壁层转移。我们讨论了Msx1在眼脉管系统中的可能功能。

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