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Characterization and comparative analyses of transcriptomes of cloned and in vivo fertilized porcine pre-implantation embryos

机译：克隆和体内受精猪植入前胚胎的转录组的表征和比较分析

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Somatic cell nuclear transfer (SCNT) is the only method known to rapidly reprogram differentiated cells into totipotent embryos. Most cloned embryos become arrested before implantation and the details of the underlying molecular mechanism remain largely unknown. Dynamic regulation of the transcriptome is a key molecular mechanism driving early embryonic development. Here, we report comprehensive transcriptomic analysis of cloned embryos (from Laiwu and Duroc pigs) andin vivofertilized embryos (from Duroc pigs) using RNA-sequencing. Comparisons between gene expression patterns were performed according to differentially expressed genes, specific-expressed genes, first-expressed genes, pluripotency genes and pathway enrichment analysis. In addition, we closely analyzed the improperly expressed histone lysine methyltransferases and histone lysine demethylases during cell reprogramming in cloned embryos. In summary, we identified altered gene expression profiles in porcine cloned pre-implantation embryos in comparison to normalin vivoembryos. Our findings provide a substantial framework for further discovery of the epigenetic reprogramming mechanisms in porcine SCNT embryos.
机译：体细胞核移植（SCNT）是已知的将分化细胞快速重编程为全能胚胎的唯一方法。大多数克隆的胚胎在植入前就被捕了，其潜在分子机制的细节在很大程度上仍然未知。转录组的动态调节是驱动早期胚胎发育的关键分子机制。在这里，我们报告使用RNA测序技术对克隆的胚胎（来自莱芜和杜洛克猪）和体内受精的胚胎（来自杜洛克猪）进行全面的转录组学分析。根据差异表达的基因，特异性表达的基因，首次表达的基因，多能性基因和途径富集分析对基因表达模式进行比较。此外，我们仔细分析了克隆胚胎中细胞重编程过程中不正确表达的组蛋白赖氨酸甲基转移酶和组蛋白赖氨酸脱甲基酶。总之，与正常体内胚胎相比，我们在猪克隆的植入前胚胎中鉴定出改变的基因表达谱。我们的发现为进一步发现猪SCNT胚胎的表观遗传重编程机制提供了一个实质性的框架。

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