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首页> 外文期刊>Clinical and Experimental Vaccine Research >Application of recombinant adenoviruses expressing glycoprotein or nucleoprotein of rabies virus to Korean raccoon dogs
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Application of recombinant adenoviruses expressing glycoprotein or nucleoprotein of rabies virus to Korean raccoon dogs

机译：表达狂犬病毒糖蛋白或核蛋白的重组腺病毒在韩国Korean狗中的应用

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Purpose A new rabies vaccine for animals, including raccoon dogs, in Korea is needed to eradicate rabies infection. In this study, we constructed two recombinant adenoviruses expressing the glycoprotein or nucleoprotein of the rabies virus (RABV). We then investigated the safety and immunogenicity of these strains in raccoon dogs, depending on inoculation route. Materials and Methods Recombinant adenoviruses expressing the glycoprotein (Ad-0910G) or nucleoprotein (Ad-0910N) of rabies were constructed in 293A cells using an adenoviral system. One-year-old raccoon dogs underwent intramuscular (IM) inoculation or oral administration of the recombinant Ad-0910G and Ad-0910N. Clinical symptoms were observed and virus-neutralizing antibodies (VNA) against RABV were measured at 0, 2, 4, and 6 weeks after the immunization. Raccoons were considered positive if VNA titers were ≥ 0.1 IU/mL. Results Raccoon dogs inoculated with the combined Ad-0910G and Ad-0910N virus via the IM route did not exhibit any clinical sign of rabies during the observation period. All raccoon dogs (n = 7) immunized IM had high VNA titers, ranging from 0.17 to 41.6 IU/mL at 2 weeks after inoculation, but 70% (7/10) of raccoon dogs administered viruses via the oral route responded by 6 weeks after administration against RABV. Conclusion Raccoon dogs inoculated with Ad-0910G and Ad-0910N viruses showed no adverse effects. Immunization with the combined Ad-0910G and Ad-0910N strains may play an important role in inducing VNA against RABV in raccoon dogs.
机译：目的需要一种在韩国用于动物（包括浣熊狗）的新狂犬病疫苗，以消除狂犬病感染。在这项研究中，我们构建了两种重组腺病毒，它们表达狂犬病毒（RABV）的糖蛋白或核蛋白。然后，我们根据接种途径调查了这些菌株在浣熊犬中的安全性和免疫原性。材料和方法使用腺病毒系统在293A细胞中构建表达狂犬病糖蛋白（Ad-0910G）或核蛋白（Ad-0910N）的重组腺病毒。一岁的浣熊犬进行了肌肉内（IM）接种或口服重组Ad-0910G和Ad-0910N。观察到临床症状，并在免疫后0、2、4和6周测量针对RABV的病毒中和抗体（VNA）。如果VNA滴度≥0.1 IU / mL，则认为浣熊呈阳性。结果在观察期间，通过IM途径接种了Ad-0910G和Ad-0910N联合病毒的浣熊狗没有狂犬病的临床症状。所有接种IM的狗（n = 7）均具有较高的VNA滴度，接种后2周的VNA滴度范围为0.17至41.6 IU / mL，但是70％（7/10）的dog狗通过口服途径给予病毒，到6周后反应对抗RABV给药后。结论接种Ad-0910G和Ad-0910N病毒的浣熊无不良反应。联合Ad-0910G和Ad-0910N菌株的免疫可能在诱导浣熊犬抗RABV的VNA诱导中起重要作用。

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