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High prevalence of Borna disease virus infection in healthy sheep in Japan.

机译:日本健康绵羊中的博尔纳病病毒感染率很高。

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Previous seroepidemiological and molecular epidemiological studies of Borna disease virus (BDV) showed considerably high rates of infection in horses, cattle, cats, and humans in Hokkaido, Japan. Here, we further demonstrate high rates of specific antibodies to BDV and BDV RNA in peripheral blood mononuclear cells (PBMCs) from healthy sheep bred on the same island. The BDV prevalences by immunoblotting and/or reverse transcriptase PCR were 0% (0 of 19) in newborns (<1 month old), 51.7% (15 of 29) in lambs (1 to 6 months old), and 36.7% (11 of 30) in adults (>2 years old). Among animals positive for BDV, 60% of lambs and 45.5% of adults contained BDV RNA in PBMCs while 46.7% of lambs and 90.9% of adults contained specific antibodies to BDV. Thus, it is suggested that virus replication in the blood, as observed in lambs, is usually reduced in adulthood by raising immune responses to BDV.
机译:先前的Borna病病毒(BDV)的血清流行病学和分子流行病学研究表明,日本北海道的马,牛,猫和人中的感染率很高。在这里,我们进一步证明了在同一岛上饲养的健康绵羊的外周血单核细胞(PBMC)中针对BDV和BDV RNA的特异性抗体的高发生率。新生儿(<1个月大)通过免疫印迹和/或逆转录酶PCR的BDV患病率分别为0%(19个中的0),羔羊(1至6个月大)中的51.7%(29个中的15个)和36.7%(11个) (30岁以上)成人(> 2岁)。在对BDV呈阳性的动物中,PBMC中60%的羔羊和45.5%的成年动物包含BDV RNA,而46.7%的羔羊和90.9%的成年动物包含针对BDV的特异性抗体。因此,建议在羔羊中观察到的血液中病毒的复制通常在成年期通过提高对BDV的免疫反应而减少。

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