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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Apoptosis in Cord Blood T Lymphocytes from Infants of Human Immunodeficiency Virus-Infected Mothers
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Apoptosis in Cord Blood T Lymphocytes from Infants of Human Immunodeficiency Virus-Infected Mothers

机译：人类免疫缺陷病毒感染母亲的婴儿脐带血T淋巴细胞的凋亡

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Apoptosis continues to be controversial in human immunodeficiency virus (HIV)-induced pathogenesis. To investigate whether apoptosis occurs with HIV exposure with or without subsequent infection, levels of apoptosis were measured in cord blood lymphocytes (CBL) from seven newborns delivered to HIV-infected mothers and seven normal, unexposed newborns. Live cells were costained with antibodies to cell surface markers and the DNA dye 7-amino actinomycin D to immunophenotype apoptotic CBL subsets. Apoptosis was measured in fresh and cultured CBL in the presence and absence of CD3 T-cell receptor stimulation. Compared to the CD4+ CBL from HIV-unexposed newborns, CD4+ CBL from six HIV-exposed, noninfected newborns demonstrated significantly greater apoptosis after overnight culture even in the absence of CD3 stimulation. Compared to HIV-unexposed controls, CD8+ CBL from the six HIV-exposed newborns also demonstrated increased levels of apoptosis after overnight culture without stimulation. The one HIV-infected newborn in this study showed the highest levels of CD4+ and CD8+ apoptotic CBL. The data suggest that levels of apoptosis are increased in infants in association with HIV infection. Furthermore, CD4+ and CD8+ cord blood lymphocytes from HIV-exposed infants behaved differently than T lymphocytes from either normal, unexposed infants or an HIV-infected infant. These results suggest that exposure to HIV or HIV-induced factors increases the levels of apoptosis in CBL.
机译：在人类免疫缺陷病毒（HIV）诱导的发病机理中，细胞凋亡仍然存在争议。为了研究是否在暴露于HIV的情况下是否发生细胞凋亡并随后感染，对分娩给受HIV感染的母亲的七个新生儿和七个未暴露的正常新生儿的脐血淋巴细胞（CBL）的细胞凋亡水平进行了测量。用针对细胞表面标志物的抗体和针对免疫表型凋亡CBL亚型的DNA染料7-氨基放线菌素D染色活细胞。在有和没有CD3 T细胞受体刺激的情况下，在新鲜和培养的CBL中测量细胞凋亡。与未接触HIV的新生儿CD4 + CBL相比，即使在没有CD3的情况下，过夜培养后，来自六个未接触HIV的HIV4新生儿的CD4 + CBL表现出明显更大的凋亡刺激。与未暴露HIV的对照相比，六个未暴露HIV的新生儿的CD8 + CBL也显示了在无刺激的情况下过夜培养后细胞凋亡的增加。在这项研究中，一个感染了HIV的新生儿显示出最高水平的CD4 + 和CD8 + 凋亡CBL。数据表明与HIV感染有关的婴儿细胞凋亡水平增加。此外，HIV感染婴儿的CD4 + 和CD8 + 脐血淋巴细胞的行为不同于正常，未暴露婴儿或HIV感染婴儿的T淋巴细胞。这些结果表明，暴露于HIV或HIV诱导的因子会增加CBL中的细胞凋亡水平。

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