Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

Franco Guscetti; Curzio Bernasconi; Kurt Tobler; Kristien Van Reeth; Andreas Pospischil; Mathias Ackermann

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Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

机译：与其他方法相比，猪流行性腹泻病毒的免疫组织化学检测

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An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.

机译：比较了使用福尔马林固定组织的免疫组织化学方法，使用低温恒温器切片的直接免疫荧光方法，酶联免疫吸附测定（ELISA）和PCR方法在经实验接种野生型断奶仔猪后的诊断结果猪流行性腹泻病毒（PEDV），并在腹泻发作后6至60小时内被杀死。免疫组织化学方法被证明与直接免疫荧光一样可靠，可用于诊断死后收集的组织中的PEDV。酶联免疫吸附法（ELISA）用于临床样品的研究具有良好的可靠性，但所用的PCR方法无效。

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《Clinical and diagnostic laboratory immunology》 |1998年第3期|共3页
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Franco Guscetti; Curzio Bernasconi; Kurt Tobler; Kristien Van Reeth; Andreas Pospischil; Mathias Ackermann;
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1. Immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (Gn) pigs infected with porcine epidemic diarrhea virus (PEDV) and serum cytokine responses of Gn pigs to acute PEDV infection [J] . Kwonil Jung, Ayako Miyazaki, Linda J. Saif Research in Veterinary Science . 2018,第期

机译：在感染猪流行性腹泻病毒（PEDV）的肠肠肠中呕吐诱导的单胺神经递质血清素和肠溶体细胞的免疫组化检测GN猪的血清细胞因子对急性PEDV感染
2. One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus [J] . Jiang Chunyan, He Haijian, Zhang Chaoying, Journal of Veterinary Diagnostic Investigation . 2019,第6期

机译：猪流行性腹泻病毒，猪腹腔病毒和猪瓣瘤病毒的一步三重逆转录PCR检测
3. Development of a duplex SYBR GreenI based real-time PCR assay for detection of porcine epidemic diarrhea virus and porcine bocavirus3/4/5 [J] . Zheng Lan-Lan, Cui Jian-Tao, Han Hao-Ying, Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 2020,第期

机译：基于双工Sybr Greeni的实时PCR测定检测猪流行性腹泻病毒和猪Bocavirus3 / 4/5的双工Sybr Greeni
4. Real-time PCR reagents for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus [C] . Christa Goodell, Lori Plourde, Kathy Velek, American Association of Swine Veterinarians Annual Meeting . 2015

机译：用于检测猪流行性腹泻病毒和猪Deltacoronavirus的实时PCR试剂
5. Evaluation of methods for inactivating porcine epidemic diarrhea virus (PEDV) in livestock trailers. [D] . Thomas, Paul R. 2015

机译：评价灭活牲畜拖车中的猪流行性腹泻病毒（PEDV）的方法。
6. Note: Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods [O] . Franco Guscetti, Curzio Bernasconi, Kurt Tobler, 1998

机译：注意：与其他方法相比猪流行性腹泻病毒的免疫组织化学检测
7. Immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (Gn) pigs infected with porcine epidemic diarrhea virus (PEDV) and serum cytokine responses of Gn pigs to acute PEDV infection [O] . Kwonil Jung, Ayako Miyazaki, Linda J. Saif 2018

机译：免疫组化检测呕吐诱导的单胺神经递质血清素和Enterochromaffin细胞在常规或侏诺菌（GN）猪的肠道中感染猪流行病毒病毒（PEDV）和GN猪的血清细胞因子对急性PEDV感染的影响
8. Compounds, Methods and Compositions Useful for the Treatment of Bovine Viral Diarrhea Virus (BVDV) Infection and Hepatitis C Virus (HCV) Infection. [R] . Dykstra, C. C., Givens, M. D., Stringfellow, D. A., 2005

机译：可用于治疗牛病毒性腹泻病毒（BVDV）感染和丙型肝炎病毒（HCV）感染的化合物，方法和组合物。

Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

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