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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela
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Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

机译：重组主要表面蛋白5用于酶联免疫吸附测定的评估，用于血清学诊断委内瑞拉牛无性病

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摘要

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of bovine anaplasmosis with purified recombinant major surface protein 5 (MSP5) of Anaplasma marginale produced in Escherichia coli. Serum antibody responses against MSP5 were detected in calves experimentally infected with A. marginale as early as 21 days postinfection and reached maximum titers at 28 days postinfection. The MSP5 ELISA performed with serum samples taken from field cattle from different regions of Venezuela showed a seroprevalence of 47%, which seems to be in accordance with the reported epidemiological status of bovine anaplasmosis in Venezuela. Positive results obtained in the MSP5 ELISA were further confirmed by immunoblotting, with the recombinant MSP5 as the antigen. Thus, these results confirmed the importance of MSP5 as a suitable antigen for the serological diagnosis of bovine anaplasmosis.
机译：建立了一种间接酶联免疫吸附试验（ELISA），用于利用大肠杆菌中生产的 Anaplasma marginale 的重组重组主表面蛋白5（MSP5）进行血清学诊断。 。在实验感染 A的犊牛中检测到针对MSP5的血清抗体反应。最早在感染后21天达到边缘，在感染后28天达到最大滴度。用从委内瑞拉不同地区的野牛采集的血清样品进行的MSP5 ELISA血清阳性率为47％，这似乎与所报道的委内瑞拉牛无性病的流行病学状况相符。以重组MSP5为抗原，通过免疫印迹进一步证实了在MSP5 ELISA中获得的阳性结果。因此，这些结果证实了MSP5作为合适的抗原用于牛血清病血清学诊断的重要性。

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