...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Apoptosis of Primary-Culture Rat Microglial Cells Induced by Pathogenic Acanthamoeba spp.
【24h】

Apoptosis of Primary-Culture Rat Microglial Cells Induced by Pathogenic Acanthamoeba spp.

机译：致病性棘阿米巴属菌种诱导的原代培养大鼠小胶质细胞的凋亡。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To determine whether trophozoites and lysates of pathogenicAcanthamoeba spp. induce apoptosis in primary-culture microglial cells, transmission electron microscopic (TEM) examinations, assessment of DNA fragmentation by agarose gel electrophoresis, and the TdT-mediated dUTP nick-end labeling assay were performed. When a trophozoite of pathogenic Acanthamoeba culbertsoni came in contact with a microglial cell, the digipodium was observed by TEM. Nuclear chromatin condensation was observed in 10% of microglial cells, while it was not revealed when they were cocultured with weakly pathogenic Acanthamoeba royreba trophozoites. DNA fragmentation in microglial cells cocultured with the A. culbertsoni lysate was detected by electrophoresis, showing DNA ladder formation, whereas it was hardly observed in microglial cells cocultured with A. royreba. DNA fragmentation of microglial cells was also confirmed by flow cytometry analysis. The fluorescence of TdT-stained apoptotic bodies became intensely visible with microglial cells cocultured with the A. culbertsoni lysate. In contrast, with microglial cells cocultured with the A. royreba lysate, only a background level of fluorescence of TdT-stained apoptotic bodies was detected. These results suggest that some rat microglial cells cocultured with pathogenic A. culbertsoni undergo cytopathic changes which show the characteristics of the apoptotic process, such as nuclear condensation and DNA fragmentation.
机译：确定是否有致病性棘阿米巴 spp的滋养体和裂解物。在原代培养的神经胶质细胞中诱导凋亡，通过透射电镜（TEM）检查，琼脂糖凝胶电泳评估DNA片段，以及TdT介导的dUTP缺口末端标记测定。当致病性棘孢棘孢子的滋养体与小胶质细胞接触时，通过透射电镜观察到了双足纲。在10％的小神经胶质细胞中观察到核染色质凝结，而与弱致病性 Acanthamoeba royreba 滋养体共培养时却没有发现。与 A共培养的小胶质细胞中的DNA断裂。电泳检测到库尔伯松溶解液，显示出DNA梯形的形成，而在与 A共培养的小胶质细胞中几乎观察不到。 royreba 。通过流式细胞术分析也证实了小胶质细胞的DNA断裂。与 A共培养的小胶质细胞强烈可见TdT染色的凋亡小体的荧光。 culbertsoni 裂解物。相反，与 A共培养的小胶质细胞。 Royreba 裂解液中，仅检测到TdT染色的凋亡小体的背景荧光水平。这些结果表明，一些大鼠小胶质细胞与致病性 A共培养。 culbertsoni 发生细胞病变，表现出凋亡过程的特征，例如核凝聚和DNA片段化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号