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Lipid Removal from Human Serum Samples

机译:从人血清样品中去除脂质

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The efficacy of lipid removal from human serum samples obtained by using Cleanascite HC, a commercially available product, was compared to that obtained by the standard chloroform method. Separate samples of 21 frozen, banked human serum samples used in the preparation of samples for proficiency testing were treated with either Cleanascite HC or chloroform. The lipid content was measured before and after treatment. The total percentages of lipid removed ranged from 61 to 70% with Cleanascite HC and from 60 to 62% with chloroform. The advantage of Cleanascite HC over chloroform is based on the simplicity of the procedure with Cleanascite HC without the environmental concerns inherent in the use of chloroform. In 15 serum samples known to contain antibodies to treponemal and nontreponemal syphilis antigens, Cleanascite HC bound some immunoglobulin, but with only minimal loss of reactivity in the serologic tests for syphilis. Cleanascite HC is therefore an acceptable alternative to chloroform for lipid reduction in human serum samples.
机译:将使用市售产品Cleanascite HC从人血清样品中去除脂质的功效与通过标准氯仿方法获得的功效进行了比较。用Cleanascite HC或氯仿处理用于制备能力验证样品的21份冷冻库存的人血清样品的单独样品。在治疗之前和之后测量脂质含量。使用Cleanascite HC去除脂质的总百分比为61%至70%,使用氯仿去除脂质的总百分比为60%至62%。 Cleanascite HC优于氯仿的优势是基于Cleanascite HC的操作简便性,而没有使用氯仿固有的环境问题。在15个已知包含针对梅毒和非梅毒抗原的抗体的血清样品中,Cleanascite HC结合了一些免疫球蛋白,但在梅毒的血清学检测中反应性的损失很小。因此,Cleanascite HC是氯仿的一种可接受的替代品,可用于降低人血清样品中的脂质。

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