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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Up-Regulation of CD40 Ligand and Induction of a Th2 Response in Children Immunized with Pneumococcal Polysaccharide Vaccines
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Up-Regulation of CD40 Ligand and Induction of a Th2 Response in Children Immunized with Pneumococcal Polysaccharide Vaccines

机译：肺炎球菌多糖疫苗免疫的儿童中CD40配体的上调和Th2反应的诱导。

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We wished to determine whether pneumococcal polysaccharide antigens induce mRNA expression of CD40 ligand (CD40L) and Th1 or Th2 cytokines in unimmunized individuals in vitro and whether immunization with the 23-valent pneumococcal polysaccharide vaccine induces changes in CD40L and cytokine mRNA expression. Children with recurrent respiratory infections were studied before and 4 to 6 weeks after receiving the pneumococcal vaccine. One patient who failed to respond to the polysacharide vaccine subsequently received a single dose of the experimental 7-valent pneumococcal conjugate vaccine. Unimmunized healthy adults were included as controls. Quantification of mRNA expression of CD40L, interleukin-4 (IL-4), IL-12p40, and gamma interferon (IFN-γ) was performed by reverse transcription-PCR and enzyme-linked immunosorbent assay (ELISA)-PCR with resting and stimulated peripheral blood mononuclear cells. Serum immunoglobulin G (IgG) anti pneumococcal antibody levels were measured by ELISA. The results showed a significant increase in the expression of mRNAs for CD40L and IL-4, but not IL-12p40 or IFN-γ, in stimulated cultures from unimmunized individuals. CD40L and IL-4 mRNA expression was significantly higher in postimmunization than in preimmunization samples stimulated with the individual pneumococcal serotypes. These results suggest that pneumococcal polysaccharide antigens specifically up-regulate CD40L expression and induce a Th2 response in vitro which parallels the increase in IgG antipneumococcal antibody levels in serum.
机译：我们希望确定肺炎球菌多糖抗原是否在体外诱导未免疫个体中CD40配体（CD40L）和Th1或Th2细胞因子的mRNA表达，以及用23价肺炎球菌多糖疫苗免疫是否诱导CD40L和细胞因子mRNA表达的变化。在接种肺炎球菌疫苗之前和之后的4至6周对患有反复呼吸道感染的儿童进行了研究。对多糖疫苗没有反应的一名患者随后接受了单剂量的实验性7价肺炎球菌结合疫苗。未免疫的健康成人被包括作为对照。通过反转录-PCR和酶联免疫吸附测定（ELISA）-PCR对CD40L，白介素4（IL-4），IL-12p40和γ干扰素（IFN-γ）的mRNA表达进行定量，并静息和刺激外周血单核细胞。通过ELISA测量血清免疫球蛋白G（IgG）抗肺炎球菌抗体水平。结果表明，在未免疫个体的刺激培养物中，CD40L和IL-4 mRNA的表达显着增加，但IL-12p40或IFN-γ却没有。免疫后，CD40L和IL-4 mRNA的表达明显高于用个别肺炎球菌血清型刺激的免疫前样品。这些结果表明，肺炎球菌多糖抗原在体外特异性上调CD40L表达并诱导Th2应答，这与血清中IgG抗肺炎球菌抗体水平的增加相平行。

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