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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antibodies to 60-Kilodalton Heat Shock Protein and Outer Membrane Protein 2 of Chlamydia pneumoniae in Patients with Coronary Heart Disease
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Antibodies to 60-Kilodalton Heat Shock Protein and Outer Membrane Protein 2 of Chlamydia pneumoniae in Patients with Coronary Heart Disease

机译：冠心病患者肺炎衣原体60-Kilodalton热休克蛋白和外膜蛋白2的抗体

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Evidence linking Chlamydia pneumoniae infection to atherosclerosis and to atherothrombotic events has recently emerged. A primary candidate implicated in these pathogenetic events is the 60-kDa chlamydial heat shock protein (HSP60). Another putative candidate to activate a potential proinflammatory mechanism is the chlamydial outer membrane protein 2 (OMP2). We have generated both HSP60 and OMP2 recombinant antigens in a nondenatured form and shown that (i) the two antigens were highly immunogenic in mice and (ii) murine antisera thus generated recognized the native C. pneumoniae proteins. We measured by enzyme linked immunosorbent assay (ELISA) and immunoblot assay antibody titers to the recombinant antigens in samples from 219 patients with coronary heart disease (CHD), 179 patients with unstable angina (UA), 40 patients with acute myocardial infarction (AMI), and 100 age-, sex-, and risk factor-matched healthy controls. We also examined whether anti-HSP60 and/or anti-OMP2 antibodies correlated with anti-C. pneumoniae antibodies assessed by a commercial microimmunofluorescence (MIF) assay. Immunoglobulin G (IgG), but neither IgA nor IgM, antibodies against the two recombinant proteins were detected by ELISA. In particular, anti-HSP60 antibodies were detected in >99% of CHD patients versus 0% of the controls, whereas the proportions of anti-OMP2 positive subjects were >70 and 27%, respectively. Nonetheless, among CHD patients, similar frequencies of positive subjects and titers of anti-HSP60 or anti-OMP2 antibodies were present in UA and AMI subjects. The anti-OMP2, but not the anti-HSP60, antibodies showed high specificity. Consistently, high serological correlation was observed between IgG MIF titers and IgG ELISA reactivity to OMP2 but not to HSP60. Overall, the results of this study demonstrate a strong correlation between CHD and anti-HSP60 IgG levels, as measured by our in-house ELISA. They also suggest that recombinant OMP2 ELISA, because of its high specificity and strong correlation with MIF assay, could be a candidate diagnostic marker for C. pneumoniae infection, which would be of potential usefulness for its specificity and nonsubjective nature.
机译：最近出现了将肺炎衣原体感染与动脉粥样硬化和动脉血栓形成事件联系起来的证据。与这些致病事件有关的主要候选药物是60 kDa衣原体热休克蛋白（HSP60）。另一个可能激活潜在促炎机制的候选人是衣原体外膜蛋白2（OMP2）。我们已经产生了非变性形式的HSP60和OMP2重组抗原，并表明（i）这两种抗原在小鼠中具有高度免疫原性，并且（ii）这样产生的鼠抗血清识别天然 C。肺炎蛋白。我们通过酶联免疫吸附测定（ELISA）和免疫印迹测定抗体滴度测定了219例冠心病（CHD），179例不稳定型心绞痛（UA），40例急性心肌梗死（AMI）患者的重组抗原，以及100个与年龄，性别和风险因素相匹配的健康对照。我们还检查了抗HSP60和/或抗OMP2抗体是否与抗 C相关。通过商业微免疫荧光（MIF）分析评估肺炎链球菌抗体。 ELISA检测到了针对两种重组蛋白的免疫球蛋白G（IgG），但未检测到IgA和IgM抗体。特别地，在> 99％的CHD患者中检测到抗HSP60抗体，而在对照中则为0％，而抗OMP2阳性受试者的比例分别> 70和27％。尽管如此，在CHD患者中，UA和AMI受试者中出现阳性受试者的频率相似，并且抗HSP60或抗OMP2抗体的滴度较高。抗OMP2抗体而非抗HSP60抗体显示出高特异性。一致地，在IgG MIF滴度和IgG ELISA对OMP2而非HSP60的反应性之间观察到高度血清学相关性。总体而言，这项研究的结果表明，通过我们的内部ELISA测量，CHD与抗HSP60 IgG水平之间存在很强的相关性。他们还建议，重组OMP2 ELISA由于其高特异性和与MIF分析的强相关性，可能成为 C的候选诊断标记。肺炎感染，对其特异性和非主观性可能具有潜在的实用性。

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