...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Surface Envelope Glycoprotein Is B-Lymphocyte Immunodominant in Sheep Naturally Infected with Ovine Progressive Pneumonia Virus
【24h】

Surface Envelope Glycoprotein Is B-Lymphocyte Immunodominant in Sheep Naturally Infected with Ovine Progressive Pneumonia Virus

机译：自然感染绵羊渐进性肺炎病毒的绵羊中的表面包膜糖蛋白是B淋巴细胞免疫抗原

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The B-lymphocyte-immunodominant antigen involved in naturally ovine progressive pneumonia virus (OPPV)-infected mature sheep remains unknown. Therefore, the amount of antibody in sera from 10 naturally OPPV-infected sheep was evaluated by immunoprecipitation (IP) of the major viral proteins in [35S]methionine/cysteine-labeled OPPV (whole virus) lysate. Using an excess of OPPV proteins in whole-virus lysate, 8 out of 10 sheep had the highest serum antibody IP endpoint titers to the gp135 surface envelope glycoprotein (SU). Also, 2 out of 10 sheep had equivalent serum antibody IP endpoint titers to the transmembrane glycoprotein oligomer (TM90) and SU. Since these data indicate that SU is the immunodominant protein in most mature sheep persistently infected with OPPV, SU-specific diagnostic serological assays can be utilized for OPPV diagnosis.
机译：参与自然绵羊进行性肺炎病毒（OPPV）感染的成熟绵羊的B淋巴细胞免疫抗原尚不清楚。因此，通过[ 35 S]蛋氨酸/半胱氨酸标记的OPPV（全病毒）裂解液中主要病毒蛋白的免疫沉淀（IP）评估了10只自然被OPPV感染的绵羊血清中抗体的量。在全病毒裂解物中使用过量的OPPV蛋白，每10只羊中就有8只对gp135表面包膜糖蛋白（SU）的血清抗体IP终点滴度最高。此外，十分之二的绵羊血清抗体IP终点滴度与跨膜糖蛋白寡聚体（TM90）和SU相当。由于这些数据表明SU是持续感染OPPV的大多数成年绵羊的免疫优势蛋白，因此SU特异性诊断血清学检测可用于OPPV诊断。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号