...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis.
【24h】

Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis.

机译：评估用于酶联免疫吸附测定的两种牛新孢子虫重组抗原，用于诊断牛新孢子虫病。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neospora caninum is a recently described apicomplexan parasite which causes paralysis and death in dogs. Neospora parasites also cause abortion and neonatal morbidity in cattle, sheep, goats, and horses, and neosporosis is emerging as an important cause of bovine abortion worldwide. The purpose of this study was to identify N. caninum cDNA clones encoding antigens that would be useful for the immunodiagnosis of bovine neosporosis. Two N. caninum tachyzoite cDNA clones expressing antigens that were recognized by serum from naturally and experimentally infected cattle were identified. The DNA sequences of these clones were determined, and the inserts were subcloned into the plasmid expression vector pTrcHisB. Both recombinant antigens, expressed as fusion proteins with a His6 tag, were purified on a nickel-chelating affinity column and evaluated in separate enzyme-linked immunosorbent assays (ELISAs). Both recombinant antigen ELISAs were capable of distinguishing between sera from Neospora-infected cows and sera from uninfected control cows. Furthermore, both assays were able to detect an antibody response in animals that were experimentally inoculated with N. caninum. Neither antigen showed evidence of cross-reactivity with serum from animals inoculated with the closely related parasites Toxoplasma gondii, Sarcocystis cruzi, Sarcocystis hominis, and Sarcocystis hirsuta.
机译：新孢子虫是最近描述的apicomplexan寄生虫，其引起狗的麻痹和死亡。新孢子虫还引起牛，羊，山羊和马的流产和新生儿发病，新孢子虫病正在成为全世界牛流产的重要原因。这项研究的目的是鉴定编码猪新孢子虫病的抗原的犬新孢子虫cDNA克隆。鉴定了两个表达抗原的犬新孢子虫速殖子cDNA克隆，该抗原被天然和实验感染牛的血清识别。确定了这些克隆的DNA序列，并将插入片段亚克隆到质粒表达载体pTrcHisB中。两种重组抗原均以带有His6标签的融合蛋白表达，并在镍螯合亲和柱上纯化，并在单独的酶联免疫吸附测定（ELISA）中进行评估。两种重组抗原ELISA都能够区分来自新孢子虫感染的母牛的血清和未感染的对照母牛的血清。此外，两种测定均能够在实验接种犬新孢子虫的动物中检测抗体应答。两种抗原均未显示与接种紧密相关寄生虫的弓形虫，克鲁氏肉胞菌，人型肉胞菌和hirscystis hirsuta的动物的血清有交叉反应的证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号