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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina
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Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

机译：阿根廷猪布鲁氏菌病血清学诊断的主要结合方法的评价

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摘要

An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.
机译：使用两个猪血清群评估了用于猪布鲁氏菌病血清学诊断的间接酶联免疫吸附测定（IELISA），竞争性ELISA（CELISA）和荧光偏振测定（FPA）：来自无布鲁氏菌病的加拿大人群的血清根据缓冲抗原板凝集试验（BPAT）和2-巯基乙醇（2-ME）试验中的阳性反应，从阿根廷选择血清和血清。此外，还评估了每个产地的成年猪血清（至少从中分离出 Brucella suis ）一次的血清。 IELISA，CELISA和FPA的特异性值分别为99.9％，99.5％和98.3％，相对于BPAT和2-ME测试的IELISA，CELISA和FPA敏感性值分别为98.9％，96.6％和93.8％ 。实际敏感性通过使用来自 B的个体猪的37份血清进行评估。培养suis ，得到的值如下：BPAT，86.5％; 2-ME测试，81.1％; IELISA，86.5％; CELISA，78.5％;和FPA为80.0％。

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