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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Optimization of the Weck-Cel Collection Method for Quantitation of Cytokines in Mucosal Secretions
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Optimization of the Weck-Cel Collection Method for Quantitation of Cytokines in Mucosal Secretions

机译：定量测定粘膜分泌物中细胞因子的Wick-Cel收集方法的优化

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Measurement of immune components in mucosal secretions is important for the evaluation of local immunity at the mucosal surfaces. The Weck-Cel ophthalmic sponge provides a method for the collection of these secretions. The sponge absorbs a relatively large volume of material, therefore allowing for quantitation of multiple immune components. Additionally, it provides a method in which the same device may be used to collect specimens from different mucosal sites, such as the genital tract and oral cavity. This sampling technique has successfully been applied for collection and measurement of antibody in oral and genital tract secretions. The purpose of this work was to optimize the extraction of protein from the sponge matrix. Of particular interest was the recovery of cytokines from the sponge. Satisfactory recovery of the cytokines interleukin 1β (IL-1β), IL-2, IL-5, IL-12, IL-6, IL-8, IL-10, and granulocyte-macrophage colony-stimulating factor was obtained. However, IL-4 and gamma interferon recovery rates remained low. Using an alteration of the published extraction method, cytokine concentrations were measured in cervical secretions from women using oral contraceptives. The data revealed detectable concentrations of IL-6, IL-10, IL-8, and IL-12 on cycle days 9 and 20. The proposed technique provides an easy, practical, and consistent method for collection of nonconventional body fluids, such as cervicovaginal fluids and saliva, for the assay of immunoglobulins and several cytokines.
机译：粘膜分泌物中免疫成分的测量对于评估粘膜表面的局部免疫性很重要。 Weck-Cel眼科海绵提供了一种收集这些分泌物的方法。海绵吸收相对大量的材料，因此可以定量多种免疫成分。另外，它提供了一种方法，其中可以使用同一设备从不同的粘膜部位（例如生殖道和口腔）收集标本。该采样技术已成功应用于口腔和生殖道分泌物中抗体的收集和测量。这项工作的目的是优化从海绵基质中提取蛋白质。特别令人感兴趣的是从海绵中回收细胞因子。获得了令人满意的细胞因子白介素1β（IL-1β），IL-2，IL-5，IL-12，IL-6，IL-8，IL-10和粒细胞巨噬细胞集落刺激因子的恢复。但是，IL-4和γ干扰素的回收率仍然很低。使用已公开的提取方法的另一种方法，使用口服避孕药测量妇女宫颈分泌物中的细胞因子浓度。数据显示在第9天和第20天可检测到IL-6，IL-10，IL-8和IL-12的浓度。所提出的技术为收集非常规体液（例如：宫颈阴道液和唾液，用于检测免疫球蛋白和几种细胞因子。

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