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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Primary Structure of the Sialodacryoadenitis Virus Genome: Sequence of the Structural-Protein Region and Its Application for Differential Diagnosis
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Primary Structure of the Sialodacryoadenitis Virus Genome: Sequence of the Structural-Protein Region and Its Application for Differential Diagnosis

机译：唾液酸腺炎病毒基因组的一级结构：结构蛋白区域的序列及其在鉴别诊断中的应用

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Sialodacryoadenitis virus (SDAV) is a coronavirus that is commonly found in laboratory rats and that causes sialodacryoadenitis and respiratory illness. We cloned and sequenced the 3′ terminal 9.8 kb of the genomic RNA and analyzed the structure of the viral genome. As with mouse hepatitis coronaviruses (MHVs), the SDAV genome was able to code for a spike protein, a small membrane protein, a membrane-associated protein, and a nucleocapsid protein. In addition, the hemagglutinin-esterase gene capable of encoding a protein of 439 amino acids (aa) was identified. The putative functional site for acetylesterase activity was present in the HE protein as Phe-Gly-Asp-Ser (FGDS), suggesting that the SDAV HE protein might have retained the esterase activity. Immediately upstream of the HE gene and downstream of the polymerase 1b gene, the NS2 nonstructural-protein gene was identified with a coding capacity of 274 aa. A motif of UCUAAAC was identified as a potential transcription signal for subgenomic mRNA synthesis. Large insertions of 172, 127, and 44 aa were detected in the N-terminal half of the predicted S protein of SDAV when its sequence was compared to the sequences of MHV 2, MHV JHM, and MHV A59, respectively. The sequence information on the SDAV S-protein gene was applied to a differential diagnostic PCR to detect and distinguish the rat coronavirus from mouse coronaviruses. This is the first report on the comprehensive genetic information of any rat coronavirus.
机译：唾液酸腺炎病毒（SDAV）是一种冠状病毒，通常在实验室大鼠中发现，会引起唾液酸腺炎和呼吸系统疾病。我们克隆并测序了基因组RNA的3'端9.8 kb，并分析了病毒基因组的结构。与小鼠肝炎冠状病毒（MHV）一样，SDAV基因组能够编码刺突蛋白，小膜蛋白，膜相关蛋白和核衣壳蛋白。另外，鉴定了能够编码439个氨基酸（aa）的蛋白质的血凝素-酯酶基因。乙酰酯酶活性的假定功能位点以Phe-Gly-Asp-Ser（FGDS）的形式存在于HE蛋白中，这表明SDAV HE蛋白可能保留了酯酶活性。在HE基因的上游和聚合酶1b基因的下游，立即鉴定出NS2非结构蛋白基因，其编码能力为274aa。 UCUAAAC的一个主题被确定为亚基因组mRNA合成的潜在转录信号。将SDAV的序列分别与MHV 2，MHV JHM和MHV A59的序列进行比较时，在SDAV的预测S蛋白的N端一半中检测到172、127和44aa的大插入。将SDAV S蛋白基因的序列信息应用于差异诊断PCR，以检测和区分大鼠冠状病毒和小鼠冠状病毒。这是关于任何大鼠冠状病毒全面遗传信息的第一份报告。

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