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首页> 外文期刊>Clinical and diagnostic laboratory immunology >2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of Enterohemorrhagic Escherichia coli Strain 4276
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2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of Enterohemorrhagic Escherichia coli Strain 4276

机译：2F3单克隆抗体识别肠出血性大肠杆菌4276菌株脂多糖的O26 O-抗原部分

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Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome in humans. Previously, it was shown that the 2F3 monoclonal antibody (MAb) is specific for the O26 EHEC and EPEC strains (P. Kerr, H. Ball, B. China, J. Mainil, D. Finlay, D. Pollock, I. Wilson, and D. Mackie, Clin. Diagn. Lab. Immunol. >6:610-614, 1999). As these groups of bacteria play an important role in pathology, the aim of this paper was to characterize the antigen recognized by the 2F3 MAb and its genetic determinant. A genomic locus containing the entire O-antigen gene cluster and half of the colanic acid gene cluster from an O26 EHEC strain was shown to be sufficient for the production of the antigen recognized by the 2F3 MAb in an E. coli DH5α strain. By transposon mutagenesis performed on the recombinant plasmid, all 2F3 enzyme-linked immunosorbent assay-negative mutants had their transposons inserted into the O-antigen gene cluster. The O-antigen gene cluster was also cloned from an O26 EHEC strain into the E. coli DH5α strain, which then produced a positive result with the 2F3 MAb. Further analysis of the type of lipopolysaccharides (smooth or rough) produced by the clones and mutants and of the O antigen of the 2F3-positive clones confirmed that the epitope recognized by the 2F3 MAb is located on the O antigen in the O26 EHEC and EPEC strains and that its genetic determinant is located inside the O-antigen gene cluster.
机译：肠出血性大肠杆菌（EHEC）和肠致病性 E。大肠埃希菌（EPEC）生物是一组致病菌株，其感染的特征是典型的肠上皮细胞附着和破损。它们参与人类和动物的肠道疾病，EHEC菌株可能导致人类溶血性尿毒症综合征。以前显示2F3单克隆抗体（MAb）对O26 EHEC和EPEC菌株具有特异性（P. Kerr，H. Ball，B.China，J.Mainil，D.Finlay，D.Pollock，I.Wilson ，和D. Mackie，临床诊断实验室免疫学杂志。> 6： 610-614，1999）。由于这些细菌在病理中起着重要作用，因此本文的目的是鉴定2F3 MAb识别的抗原及其遗传决定因素。已显示包含完整的O抗原基因簇和来自O26 EHEC菌株的可乐酸基因簇的一半的基因组基因座足以在em中产生2F3 MAb识别的抗原。大肠杆菌DH5α菌株。通过对重组质粒进行转座子诱变，所有2F3酶联免疫吸附测定阴性的突变体都将其转座子插入到O抗原基因簇中。还从O26 EHEC菌株中将O抗原基因簇克隆到 E中。大肠杆菌DH5α菌株，然后用2F3 MAb产生阳性结果。进一步分析克隆和突变体产生的脂多糖的类型（光滑或粗糙）以及2F3阳性克隆的O抗原，证实2F3 MAb识别的表位位于O26 EHEC和EPEC中的O抗原上菌株，并且其遗传决定因素位于O抗原基因簇内部。

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