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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens.
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Diagnostic value of the indirect immunofluorescence assay in cat scratch disease with Bartonella henselae and Afipia felis antigens.

机译：间接免疫荧光测定法对亨氏巴尔通体和猫非阿非斯抗原在猫抓病中的诊断价值。

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Serum samples from 35 cat scratch disease (CSD) patients, 180 control patients (123 without lymph node enlargement and 57 with lymph node enlargement not evoking CSD), and 102 nonpatient subjects (35 with cat contact and 67 without cat contact) were tested by semiquantitative indirect immunofluorescence assay for the presence of antibodies directed to Afipia felis (ATCC 53690T) or Bartonella henselae (ATCC 49882T). The CSD group had statistically higher antibody titers against B. henselae than the control groups (P < 10(-5)), whereas no difference in A. felis antibody titers was evidenced among all groups tested. Among the 317 serum samples studied, the three with high A. felis antibody titers ( > or = 64) also had high antibody titers against other alpha-2 proteobacteria. The value of the indirect immunofluorescence assay with B. henselae antigen for the diagnosis of CSD was as follows: for a cutoff of 32, sensitivity was 0.80, specificity was 0.85, and the likelihood ratio was 5.1; for a cutoff of 64, the likelihood ratio was 12.1. In summary, in France, CSD is associated with high antibody titers against B. henselae, as previously described in the United States. However, the causes for B. henselae seronegativity in CSD patients and those for high antibody titers outside the typical nosological frame of CSD still have to be identified.
机译：通过以下方法测试了35名猫抓挠性疾病（CSD）患者，180名对照患者（123名无淋巴结肿大和57名淋巴结肿大而不引起CSD）和102名非患者受试者（35名有猫接触和67名无猫接触）的血清样本。半定量间接免疫荧光测定法，用于检测针对猫阿非比氏菌（ATCC 53690T）或汉氏巴尔通体（ATCC 49882T）的抗体。在统计学上，CSD组对汉森芽孢杆菌的抗体滴度高于对照组（P <10（-5）），而在所有测试组中均未发现猫屎曲霉抗体滴度有差异。在研究的317个血清样品中，三个具有较高曲霉抗体滴度（>或= 64）的样品也具有针对其他alpha-2蛋白质细菌的高抗体滴度。用汉氏芽孢杆菌抗原进行间接免疫荧光测定对CSD的诊断价值如下：截止值为32，灵敏度为0.80，特异性为0.85，似然比为5.1。截止值为64，则似然比为12.1。总之，在法国，如先前在美国所述，CSD与抗汉森芽孢杆菌的高抗体滴度有关。但是，仍需查明CSD患者中汉森芽孢杆菌血清阴性的原因以及典型CSD病原学框架之外高抗体滴度的原因。

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