Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

R R Wittler; M M Lieberman; D D Paine; S L Muehlbauer; J E Lima; D M Sachanandani

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Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

机译：在新生和成年大鼠多形核白细胞上γ干扰素预孵育的化学发光和流式细胞术分析。

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Gamma interferon (IFN-gamma) has multiple immunomodulating effects and has been postulated as a possible immunopotentiating agent for the prevention or treatment of neonatal infections. This report describes the effect of rat recombinant IFN-gamma on the oxidative burst activity and CD11b expression of neonatal and adult rat polymorphonuclear leukocytes (PMNL). Oxidative burst activity was assessed by chemiluminescence and dihydrorhodamine flow cytometry. Neonatal PMNL exhibited significantly less oxidative burst activity than did adult PMNL. IFN-gamma mildly enhanced the chemiluminescence response of PMNL from both the rat pups and adults, but this effect was not statistically significant when analyzed by a multivariate model of repeated-measures analysis of variance for both chemiluminescence and dihydrorhodamine flow cytometry. CD11b expression was also not significantly enhanced by IFN-gamma.

机译：γ干扰素（IFN-γ）具有多种免疫调节作用，被认为是预防或治疗新生儿感染的可能的免疫增强剂。该报告描述了大鼠重组IFN-γ对新生和成年大鼠多形核白细胞（PMNL）的氧化爆发活性和CD11b表达的影响。通过化学发光和二氢罗丹明流式细胞仪评估氧化爆发活性。新生儿PMNL的氧化爆发活性明显低于成人PMNL。 IFN-γ温和地增强了来自幼鼠和成年小鼠的PMNL的化学发光反应，但是当通过化学发光和二氢罗丹明流式细胞术的方差的重复测量多变量模型进行分析时，这种效果在统计学上并不显着。 CD11b表达也没有被IFN-γ显着增强。

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《Clinical and diagnostic laboratory immunology》 |1996年第5期|共6页
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R R Wittler; M M Lieberman; D D Paine; S L Muehlbauer; J E Lima; D M Sachanandani;
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Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

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