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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of candidal antigens in autoimmune polyglandular syndrome type I.
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Detection of candidal antigens in autoimmune polyglandular syndrome type I.

机译：检测自身免疫性I型多腺综合征的念珠菌抗原

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Autoimmune polyglandular syndrome type I (APS I) is associated with chronic mucocutaneous candidiasis. To characterize the antibody responses in this subgroup of Candida albicans infections, we screened a candidal cDNA expression library with patient sera and found four cDNA clones encoding the immunopositive proteins enolase, heat shock protein 90, pyruvate kinase, and alcohol dehydrogenase. The reactivity to these antigens was studied further by immunoprecipitation assays with in vitro-transcribed and -translated proteins. Analysis of sera from 44 APS I patients showed that the highest antibody reactivity was found with enolase (80% of patients reactive), but significant serological responses were also found with heat shock protein 90 (67%), pyruvate kinase (62.5%), and alcohol dehydrogenase (64%). Overall, 95.5% of patients had detectable antibodies to at least one of these proteins. The cDNAs of enolase and heat shock protein 90 were also expressed in Escherichia coli and studied by immunoblotting. Again, 84% of sera reacted with enolase, whereas 44% of sera reacted with heat shock protein 90. A good correlation between the two methods was found for both enolase (r = 0.86; n = 58; P < 0.001) and heat shock protein 90 (r = 0.71; n = 56; P < 0.001). Our results indicate that the four abundant candidal proteins are the major antigens and can be used as accurate markers of candidiasis in APS I patients. The immunoprecipitation assay described here is particularly useful for the rapid analysis of a large number of samples.
机译：I型自身免疫性多腺综合征（APS I）与慢性粘膜皮肤念珠菌病有关。为了表征该白色念珠菌感染亚组中的抗体应答，我们筛选了具有患者血清的念珠菌cDNA表达文库，发现了四个编码免疫阳性蛋白烯醇酶，热休克蛋白90，丙酮酸激酶和酒精脱氢酶的cDNA克隆。通过体外转录和翻译的蛋白的免疫沉淀测定进一步研究了对这些抗原的反应性。对44例APS I患者的血清分析表明，烯醇酶发现抗体反应性最高（80％的患者具有反应性），但热休克蛋白90（67％），丙酮酸激酶（62.5％），血清学反应也很明显，和酒精脱氢酶（64％）。总体而言，95.5％的患者具有针对这些蛋白质中至少一种的可检测抗体。烯醇酶和热休克蛋白90的cDNA在大肠杆菌中也表达，并通过免疫印迹进行研究。同样，84％的血清与烯醇酶反应，而44％的血清与热激蛋白90反应。两种方法在烯醇酶（r = 0.86; n = 58; P <0.001）和热休克中均发现了良好的相关性。蛋白质90（r = 0.71; n = 56; P <0.001）。我们的结果表明，四种丰富的念珠菌蛋白是主要抗原，可以用作APS I患者念珠菌病的准确标记。本文所述的免疫沉淀测定法对于大量样品的快速分析特别有用。

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