...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Phagocytosis and oxidative-burst response of planktonic Staphylococcus epidermidis RP62A and its non-slime-producing variant in human neutrophils.
【24h】

Phagocytosis and oxidative-burst response of planktonic Staphylococcus epidermidis RP62A and its non-slime-producing variant in human neutrophils.

机译：嗜中性粒细胞中浮游性表皮葡萄球菌RP62A及其非粘液变体的吞噬作用和氧化爆发反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ability of bacterial organisms to produce an extracellular polysaccharide matrix known as slime has been associated with increased virulence and delayed infections in various prosthetic implants. Within a biofilm, this slime may protect the embedded bacteria from host defense mechanisms, especially phagocytosis by polymorphonuclear leukocytes. To determine whether planktonic Staphylococcus epidermidis is protected in a similar way, a novel flow cytometric assay was performed, measuring ingestion and adherence during phagocytosis and the production of superoxide during oxidative burst. Hydrophobicity was determined by hydrophobic interaction chromatography. Slime-producing S. epidermidis RP62A and its phenotypic variant, non-slime-producing RP62A-NA, were compared. The results showed increased phagocytosis of RP62A at 2, 5, 10, and 30 min; increased adherence of RP62A at 30 s and 30 min; and increased superoxide production of RP62A after 2 min. Decreased hydrophobicity of RP62A over RP62A-NA was correlated with a hydrophilic slime coat. The data argue that the host aggressively combats slime-producing S. epidermidis. This biological phenomenon is potentially important during bacteremia to prevent further adhesion, accumulation, and the genesis of a bacterial biofilm on implants or tissue surfaces.
机译：细菌生物体产生称为粘液的细胞外多糖基质的能力与各种假体植入物中毒力的提高和感染的延迟有关。在生物膜内，这种粘液可以保护嵌入的细菌免受宿主防御机制的侵害，尤其是多形核白细胞的吞噬作用。为了确定浮游性表皮葡萄球菌是否以类似的方式受到保护，进行了一种新颖的流式细胞术测定，测量吞噬作用期间的摄入和粘附以及氧化爆发过程中超氧化物的产生。通过疏水相互作用色谱法测定疏水性。比较了产生粘液的表皮葡萄球菌RP62A及其表型变体，没有产生粘液的RP62A-NA。结果显示，在2、5、10和30分钟时，RP62A的吞噬作用增加;在30秒和30分钟时增加了RP62A的依从性; 2分钟后，RP62A的超氧化物产量增加。 RP62A相对于RP62A-NA的疏水性降低与亲水性粘液涂层有关。数据表明，宿主积极地对抗产生粘液的表皮葡萄球菌。这种生物现象在菌血症期间对防止进一步的附着，积累和植入物或组织表面细菌生物膜的产生具有潜在的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号