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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Rapid Detection of Antigenic Diversity of Akabane Virus Isolates by Dot Immunobinding Assay Using Neutralizing Monoclonal Antibodies
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Rapid Detection of Antigenic Diversity of Akabane Virus Isolates by Dot Immunobinding Assay Using Neutralizing Monoclonal Antibodies

机译：使用中和性单克隆抗体的点免疫结合法快速检测赤羽病毒分离物的抗原多样性

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Akabane (AKA) virus is an arthropod-borne virus belonging to the Simbu group of the genus Bunyavirus. Neutralizing monoclonal antibodies (MAbs) against AKA virus were prepared, and the neutralizing epitopes of the virus were defined by competitive binding assay. Five distinct antigenic domains were identified and were designated A, B, C, D, and E. Domains A and C consisted of two epitopes each. It was demonstrated that seven neutralizing epitopes exist on the G1 glycoprotein of AKA virus. Dot immunobinding assays (DIAs) were performed with MAbs which recognize these seven neutralizing epitopes. The results were similar to those obtained by enzyme-linked immunosorbent assay. DIAs were performed using two Australian strains, one isolate from Taiwan, and isolates from Japan collected between the years 1959 and 1994, for a total of 63 isolates. The MAb response patterns were divided into five groups: the OBE-1 strain, the JaGAr39 strain, the Iriki strain, a group which consisted of features between those of the JaGAr39 strain and Iriki strain groups, and a group which did not belong to any of these patterns. The isolates which showed patterns similar to that of the JaGAr39 strain were found mostly among the isolates collected in 1974 and 1990, and isolates with patterns of MAb responses similar to the pattern of the Iriki strain were found mostly in the 1985 isolates. Those showing patterns in between were found mostly around 1977, 1987, and 1994. The results show that DIA can be used to effectively compare the antigenicities of AKA virus isolates within a few hours, even with lesser amounts of virus culture than is required for other assays.
机译：赤羽（AKA）病毒是节肢动物传播的病毒，属于 Bunyavirus 属Simbu组。制备了抗AKA病毒的中和单克隆抗体（MAbs），并通过竞争结合试验确定了该病毒的中和表位。鉴定出五个不同的抗原结构域，分别命名为A，B，C，D和E。结构域A和C分别由两个表位组成。证实在AKA病毒的G1糖蛋白上存在七个中和表位。用识别这七个中和表位的单克隆抗体进行点免疫结合测定（DIAs）。结果类似于通过酶联免疫吸附测定获得的结果。 DIAs使用两种澳大利亚菌株进行分离，其中一种来自台湾，另一种来自日本，分别于1959年至1994年间收集，共63种。 MAb反应模式分为五组：OBE-1菌株，JaGAr39菌株，Iriki菌株，由JaGAr39菌株和Iriki菌株组之间的特征组成的组，以及不属于任何组的一组这些模式中。在1974年和1990年收集的菌株中发现的菌株与JaGAr39菌株相似，而在1985年的菌株中发现的MAb反应菌株与Iriki菌株相似。那些表现出这种模式的病毒大多在1977、1987和1994年左右发现。结果表明，DIA可以在几个小时内有效比较AKA病毒分离株的抗原性，即使病毒培养量比其他方法少分析。

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