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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Molecular Cloning and Nucleotide Sequence Analysis of a Novel Human Papillomavirus (Type 82) Associated with Vaginal Intraepithelial Neoplasia
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Molecular Cloning and Nucleotide Sequence Analysis of a Novel Human Papillomavirus (Type 82) Associated with Vaginal Intraepithelial Neoplasia

机译：新型人类乳头瘤病毒（类型82）与阴道上皮内瘤变相关的分子克隆和核苷酸序列分析

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摘要

The genome of a novel human papillomavirus (HPV-82) was cloned from a vaginal intraepithelial neoplasia grade I. In our series of 291 biopsy specimens, HPV-82 was identified in one case each of cervical intraepithelial neoplasia grade II and grade III by blot hybridization. The histological localization of HPV-82 DNA in the three lesions was confirmed by in situ hybridization. The results indicated that HPV-82 is an etiologic agent for vaginal and cervical intraepithelial neoplasia. By nucleotide sequence similarity of L1 open reading frame (ORF), HPV-82 was closely related to HPV-26, -51, and -69. To know the precise relationship between the HPVs, we determined the complete sequence of HPV-82, as well as that of HPV-69. Sequencing revealed that the four HPVs had no initiation codon in the E5 ORF and had extensive nucleotide sequence similarities in all ORFs. In addition, they exhibited unique frame position patterns for ORFs, different from those of the other genital HPVs.
机译：从阴道上皮内瘤样增生I级克隆了新型人乳头瘤病毒（HPV-82）的基因组。在我们的291份活检标本系列中，通过印迹法在每例宫颈上皮内瘤变II级和III级中鉴定出HPV-82杂交。通过原位杂交证实HPV-82 DNA在三个病变中的组织学定位。结果表明HPV-82是阴道和宫颈上皮内瘤变的病原体。通过L1开放阅读框（ORF）的核苷酸序列相似性，HPV-82与HPV-26，-51和-69密切相关。要知道HPV之间的确切关系，我们确定了HPV-82以及HPV-69的完整序列。测序显示，四个HPV在E5 ORF中没有起始密码子，并且在所有ORF中都具有广泛的核苷酸序列相似性。此外，它们显示出ORF独特的框架位置模式，与其他生殖器HPV的不同。

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