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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Down Regulation of CD4 Expression following Isolation and Culture of Human Monocytes
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Down Regulation of CD4 Expression following Isolation and Culture of Human Monocytes

机译：人类单核细胞分离培养后CD4表达的下调

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The down regulation of CD4 by cultured monocytes has been observed by our group and by other investigators. Flow cytometric experiments were done to examine which factors might influence this phenomenon. The addition of lipopolysaccharide, granulocyte-macrophage colony-stimulating factor, macrophage colony-stimulating factor, or interleukin-10 to monocyte cultures failed to inhibit the decrease in monocyte CD4 expression routinely observed following overnight culture. The down regulation was an adherence-independent phenomenon and was not influenced by the type of anticoagulant into which the peripheral blood was collected or by the presence or absence of lymphocytes within the cultures. The avoidance of the use of Ficoll-Paque to isolate peripheral blood mononuclear cells did not prevent monocyte CD4 down regulation. Finally, by tagging monocyte CD4 with an anti-CD4 phycoerythrin-conjugated monoclonal antibody prior to culture, we were able to determine that the down regulation observed was the result of the internalization of the molecule. At this time, we conclude that the observed down regulation of monocyte CD4 is probably due to the differentiation of blood monocytes into tissue culture-derived macrophages rather than to some artifact of the isolation procedure.
机译：我们小组和其他研究者已经观察到培养的单核细胞对CD4的下调。进行了流式细胞术实验，以检查哪些因素可能影响此现象。向单核细胞培养物中添加脂多糖，粒细胞巨噬细胞集落刺激因子，巨噬细胞集落刺激因子或白细胞介素10不能抑制过夜培养后常规观察到的单核细胞CD4表达的降低。下调是不依赖于粘附的现象，并且不受收集外周血的抗凝剂类型或培养物中淋巴细胞的存在与否的影响。避免使用Ficoll-Paque分离外周血单核细胞不会阻止单核细胞CD4下调。最后，通过在培养之前用抗CD4藻红蛋白偶联的单克隆抗体标记单核细胞CD4，我们能够确定观察到的下调是分子内在化的结果。这时，我们得出结论，观察到的单核细胞CD4的下调可能是由于血液单核细胞分化成组织培养衍生的巨噬细胞，而不是由于分离过程的某些伪影。

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