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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of Chlamydia pneumoniae 43- and 53-Kilodalton Recombinant Proteins for Serodiagnosis by Western Blot
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Evaluation of Chlamydia pneumoniae 43- and 53-Kilodalton Recombinant Proteins for Serodiagnosis by Western Blot

机译：Western blot评估肺炎衣原体43-和53-Kilodalton重组蛋白在血清学诊断中的作用

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Chlamydia pneumoniae is a common cause of respiratory infection. It has also been shown to be associated with coronary heart disease. Two proteins that have been reported to be recognized frequently during human infection are proteins having molecular masses of 43 and 53 kDa. In order to develop a useful alternative serological test to the microimmunofluorescence (micro-IF) assay, recombinant 43-kDa and 53-kDa chlamydia-specific proteins were evaluated in dot blot and/or for comparison to the standard micro-IF test. Primers for amplification were derived from genome sequence information for two C. pneumoniae genes (CPn0809 and CPn0980) encoding 53-kDa proteins and four C. pneumoniae genes (CPn0562, CPn0927, CPn0928, and Cpn0929) encoding 43-kDa proteins of unknown function, which wereChlamydia specific and not found in Chlamydia trachomatis. The 53-kDa protein product of CPn0809 or the N-terminal 18-kDa portion had better specificity than any of the 43-kDa recombinants but was much less sensitive than micro-IF. In contrast, the 53-kDa protein encoded by CPn0980 was recognized by 11 of 12 (92%) acute-phase sera, 35 of 46 (76%) chronic sera, 0 of 12 micro-IF-negative sera (C. pneumoniae andC. trachomatis negative), and 1 of 12 (8%)C. pneumoniae negative, C. trachomatis positive sera. Thus, it appears that the 53-kDa protein encoded by CPn0980 has potential use for serodiagnosis ofC. pneumoniae infection.
机译：肺炎衣原体是呼吸道感染的常见原因。它也被证明与冠心病有关。据报道，在人类感染期间经常被识别的两种蛋白质是分子量为43和53 kDa的蛋白质。为了开发一种有用的替代性血清学检测方法来代替微免疫荧光（micro-IF）检测，在斑点印迹中评估了重组43-kDa和53-kDa衣原体特异性蛋白，和/或与标准micro-IF测试进行了比较。用于扩增的引物来自两个C的基因组序列信息。肺炎基因（CPn0809和CPn0980）编码53-kDa蛋白和4个 C。肺炎基因（CPn0562，CPn0927，CPn0928和Cpn0929）编码43-kDa功能未知的蛋白，这些蛋白具有衣原体特异性，但在沙眼衣原体中找不到。 CPn0809的53 kDa蛋白质产物或N端18 kDa部分比任何43 kDa重组体具有更好的特异性，但敏感性不如micro-IF。相比之下，CPn0980编码的53 kDa蛋白被12个急性期血清中的11个（占92％），46个慢性病血清中的35个（占76％），12个微IF阴性血清中的0个被识别（ C肺炎和沙眼衣原体阴性）和12分之一（8％） C。肺炎阴性， C。沙眼阳性血清。因此，似乎由CPn0980编码的53-kDa蛋白具有潜在的用于 C的血清诊断的用途。肺炎感染。

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