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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Use of Recombinant BP26 Protein in Serological Diagnosis of Brucella melitensis Infection in Sheep
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Use of Recombinant BP26 Protein in Serological Diagnosis of Brucella melitensis Infection in Sheep

机译：重组BP26蛋白在绵羊布鲁氏菌感染血清学诊断中的应用

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摘要

Previously a Brucella protein named CP28, BP26, or Omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. In the present study we evaluated antibody responses of infected and B. melitensisRev.1-vaccinated sheep to the BP26 protein using purified recombinant BP26 protein produced in Escherichia coli in an indirect enzyme-linked immunosorbent assay (I-ELISA). The specificity of the I-ELISA determined with sera from healthy sheep (n = 106) was 93%. The sensitivity of the I-ELISA assessed with sera from naturally infected and suspected sheep found positive in the current conventional diagnostic tests was as follows: 100% for bacteriologically and serologically positive sheep (n = 50), 88% for bacteriologically negative but serologically and delayed-type hypersensitivity-positive sheep (n = 50), and 84% for bacteriologically and serologically negative but delayed-type hypersensitivity-positive sheep (n = 19). However, the absorbance values observed did not reach those observed in an I-ELISA using purified O-polysaccharide (O-PS) as an antigen. In sheep experimentally infected with B. melitensis H38 the antibody response to BP26 was delayed and much weaker than that to O-PS. Nevertheless, the BP26 protein appears to be a good diagnostic antigen to be used in confirmatory tests and for serological differentiation between infected and B. melitensis Rev.1-vaccinated sheep. Weak antibody responses to BP26 in some of the latter sheep suggest that aB. melitensis Rev.1 bp26 gene deletion mutant should be constructed to ensure this differentiation.
机译：以前，一种名为CP28，BP26或Omp28的 Brucella 蛋白已被确定为感染牛，羊，山羊和人类中的一种免疫优势抗原。在本研究中，我们评估了感染的和 B的抗体反应。使用间接酶联免疫吸附试验（I-ELISA），使用在大肠杆菌中产生的纯化的重组BP26蛋白，将经Melitensis Rev.1接种的绵羊接种到BP26蛋白上。用健康绵羊（ n = 106）的血清测定的I-ELISA的特异性为93％。在当前常规诊断测试中，发现自然感染和可疑绵羊呈阳性的血清对I-ELISA的敏感性如下：细菌学和血清学呈阳性的绵羊（ n = 50）为100％，88细菌学阴性但血清学和迟发型超敏反应阳性的绵羊（ n = 50）的百分比为84％，细菌学和血清学阴性但药后迟发型超敏反应阳性的绵羊（ n em> = 19）。但是，使用纯化的O-多糖（O-PS）作为抗原的I-ELISA中观察到的吸光度值未达到。在实验中感染 B的绵羊中。 H38抗体对BP26的反应被延迟并且比对O-PS的反应弱得多。尽管如此，BP26蛋白似乎是一种很好的诊断抗原，可用于确认性测试以及感染和B的血清学区分。已接种疫苗的羊群。在后者的一些绵羊中对BP26的抗体应答较弱表明aemB。应该构建melitensis Rev.1 bp26 基因缺失突变体以确保这种分化。

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