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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Enzyme-Linked Immunosorbent Assay for Recombinant K39 Antigen in Diagnosis and Prognosis of Indian Visceral Leishmaniasis
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Enzyme-Linked Immunosorbent Assay for Recombinant K39 Antigen in Diagnosis and Prognosis of Indian Visceral Leishmaniasis

机译：酶联免疫吸附测定重组K39抗原在印度内脏利什曼病的诊断和预后中的作用

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摘要

The recombinant product (rK39) of the 39-amino-acid repeats encoded by a kinesin-like protein-encoding gene of Leishmania chagasi was evaluated by enzyme-linked immunosorbent assay (ELISA) for diagnostic potential and the ability to predict the response to therapy in Indian kala-azar or visceral leishmaniasis (VL); we also compared its performance with that of crude soluble antigen (CSA). At the diagnosis of VL, the anti-rK39 antibody titer was 59-fold higher than the anti-CSA antibody titer. With successful therapy, antibody titers declined steeply at the end of treatment and during follow-up. In contrast, patients who relapsed showed increased titers of antibodies to rK39. The extremely high levels of anti-rK39 antibodies in VL cases suggest the application of rK39 for sensitive and specific serodiagnosis, and rK39 ELISA is also valuable in monitoring drug therapy and detecting relapse of the disease.
机译：用酶联免疫吸附试验（ELISA）评估了由 Leishmania chagasi 的一种类似驱动蛋白的蛋白编码基因编码的39个氨基酸重复序列的重组产物（rK39）的诊断潜力，并通过能够预测印度黑热病或内脏利什曼病（VL）对治疗反应的能力;我们还比较了其与粗制可溶性抗原（CSA）的性能。诊断为VL时，抗rK39抗体滴度比抗CSA抗体滴度高59倍。通过成功的治疗，抗体滴度在治疗结束时和随访期间急剧下降。相反，复发的患者显示抗rK39抗体滴度增加。 VL病例中极高水平的抗rK39抗体表明rK39在敏感和特异性血清诊断中的应用，rK39 ELISA在监测药物治疗和检测疾病复发方面也很有价值。

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