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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Increased Production of Proinflammatory Cytokines following Infection with Porcine Reproductive and Respiratory Syndrome Virus and Mycoplasma hyopneumoniae
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Increased Production of Proinflammatory Cytokines following Infection with Porcine Reproductive and Respiratory Syndrome Virus and Mycoplasma hyopneumoniae

机译：猪繁殖与呼吸综合征病毒和猪肺炎支原体感染后促炎细胞因子的产量增加

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Induction of the proinflammatory cytokines interleukin-1 (IL-1) (α and β), IL-6, IL-8, IL-10, IL-12, and tumor necrosis factor alpha (TNF-α) in pulmonary alveolar macrophages (PAMs) was assessed following experimental infection with porcine reproductive and respiratory syndrome virus (PRRSV) and/or Mycoplasma hyopneumoniae by using in vivo and in vitro models. The in vivo model consisted of pigs infected with PRRSV and/or M. hyopneumoniae and necropsied at 10, 28, or 42 days postinfection. Pigs infected with both pathogens had a greater percentage of macroscopic lung lesions, increased clinical disease, and slower viral clearance than pigs infected with either pathogen alone. The pigs infected with both PRRSV and M. hyopneumoniae had significantly increased levels of mRNA for many proinflammatory cytokines in PAMs collected by bronchoalveolar lavage (BAL) at all necropsy dates compared to those in uninfected control pigs. Increased levels of IL-1β, IL-8, IL-10, and TNF-α proteins in BAL fluid, as measured by enzyme-linked immunosorbent assay, confirmed the increased cytokine induction induced by the pathogens. An in vitro model consisted of M. hyopneumoniae-inoculated tracheal ring explants cultured with PRRSV-infected PAMs. PAMs were harvested at 6 or 15 h postinfection with either or both pathogens. The in vitro study detected increased IL-10 and IL-12 mRNA levels in PAMs infected with PRRSV at all time periods. In addition, IL-10 protein levels were significantly elevated in the culture supernatants in the presence of M. hyopneumoniae-inoculated tracheal ring explants. The increased production of proinflammatory cytokines in vivo and in vitro associated with concurrent M. hyopneumoniae and PRRSV infection may play a role in the increased rates of pneumonia associated with PRRSV infection. The increased levels of IL-10 may be a possible mechanism that PRRSV and M. hyopneumoniae use to exacerbate the severity and duration of pneumonia induced by PRRSV and modulate the respiratory immune response.
机译：肺泡巨噬细胞中促炎细胞因子白介素-1（IL-1）（α和β），IL-6，IL-8，IL-10，IL-12和肿瘤坏死因子α（TNF-α）的诱导（使用体内和体外模型，对猪繁殖与呼吸综合症病毒（PRRSV）和/或猪肺炎支原体进行实验性感染后评估PAMs。体内模型由感染PRRSV和/或 M的猪组成。感染后10、28或42天进行尸检。与仅感染任何一种病原体的猪相比，感染这两种病原体的猪具有更大的宏观肺损伤百分比，临床疾病增加，病毒清除速度较慢。感染PRRSV和 M的猪。与未感染对照猪相比，在所有尸检日期，猪肺炎支气管肺泡灌洗（BAL）收集的PAM中许多促炎细胞因子的mRNA水平均显着升高。通过酶联免疫吸附法测定，BAL液中IL-1β，IL-8，IL-10和TNF-α蛋白的水平升高，证实了病原体诱导的细胞因子诱导增加。体外模型由 M组成。猪肺炎支原体接种PRRSV感染的PAMs。在感染后6或15小时收获一种或两种病原体的PAM。体外研究在所有时间段均检测到感染PRRSV的PAM中IL-10和IL-12 mRNA水平升高。另外，在 M的存在下，培养上清液中IL-10蛋白水平显着升高。猪肺炎链球菌外植体。与并发M相关的体内和体外促炎细胞因子产量的增加。肺炎链球菌和PRRSV感染可能与PRRSV感染相关的肺炎发病率增加有关。 IL-10水平升高可能是PRRSV和 M的可能机制。猪肺炎用于加剧PRRSV诱发的肺炎的严重程度和持续时间，并调节呼吸道免疫反应。

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