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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Immunoreactivity of Five Monoclonal Antibodies against the 37-Kilodalton Common Cell Wall Protein (PsaA) ofStreptococcus pneumoniae
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Immunoreactivity of Five Monoclonal Antibodies against the 37-Kilodalton Common Cell Wall Protein (PsaA) ofStreptococcus pneumoniae

机译：五个单克隆抗体对肺炎链球菌的37 Kilodalton共有细胞壁蛋白（PsaA）的免疫反应

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Five monoclonal antibodies (MAbs) were produced against theStreptococcus pneumoniae pneumococcal surface adhesin A (PsaA) 37-kDa common cell wall protein. These antibodies were used in a dot immunoblot and Western blot study of clinical isolates ofS. pneumoniae to detect the presence of the protein. By both assays, the MAbs reacted with clinical isolates representing the 23 type-specific serotypes present in the licensed pneumococcal polysaccharide vaccine. Western blot analysis confirmed the presence of a protein migrating in the gel with a molecular mass of 37 kDa. An extension of the study by using dot immunoblot analysis that included an analysis of the 90 serotypes of S. pneumoniae showed that all five MAbs reacted with 89 of the 90 serotypes tested. MAb 1B6, the exception, did not react with S. pneumoniae serotype 16F. Dot immunoblot analysis of the MAbs with Enterococcus faecalis and viridans streptococci showed varied reactivity patterns, depending on the species. The MAbs against the 37-kDa antigen did not react with Escherichia coli, respiratory pathogens, or nonpathogens representing 22 genera and 29 species of bacteria. All five MAbs also reacted with five multidrug-resistant strains ofS. pneumoniae. In summary, these MAbs may be useful for detection of pneumococcal antigen and may lead to the development of diagnostic assays for pneumococcal disease.
机译：针对肺炎链球菌肺炎球菌表面粘附素A（PsaA）37-kDa常见细胞壁蛋白，生产了5种单克隆抗体（MAb）。这些抗体用于 S临床分离株的斑点免疫印迹和蛋白质印迹研究。肺炎来检测蛋白质的存在。通过两种测定，MAb与代表许可的肺炎球菌多糖疫苗中存在的23种类型特异性血清型的临床分离物反应。 Western印迹分析证实了存在于分子中的分子量为37kDa的蛋白质迁移。通过使用点免疫印迹分析扩大研究范围，其中包括对90种血清型 S的分析。肺炎表明，所有五种单克隆抗体均与90种血清型中的89种发生了反应。例外，单克隆抗体1B6对 S没有反应。血清型16F的肺炎。粪肠球菌和绿链球菌链球菌对MAb的斑点免疫印迹分析显示出不同的反应模式，具体取决于物种。抗37 kDa抗原的单克隆抗体不与代表22属29种细菌的大肠埃希氏菌，呼吸道病原体或非病原体反应。所有五种单克隆抗体也与五种对 S具有多重耐药性的菌株反应。肺炎。总之，这些MAb可用于检测肺炎球菌抗原，并可导致针对肺炎球菌疾病的诊断测定方法的发展。

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