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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to O157 Antigen ofEscherichia coli
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Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to O157 Antigen ofEscherichia coli

机译：用于检测大肠杆菌O157抗原血清抗体的封闭酶联免疫吸附法的开发

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The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica O9, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with E. coli O157:H7 LPS as the antigen and a monoclonal antibody specific for E. coli O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera from naive heifers vaccinated for or experimentally infected with B. abortus had increased anti-O157 bELISA titers, while 61% of anti-O157 iELISA titers were increased. The bELISA is a sensitive and specific method for the detection of serum antibodies resulting from exposure to E. coli O157.
机译：大肠埃希氏菌的O157抗原与其他细菌的脂多糖（LPS）抗原共有结构元素，特别是流产布鲁氏菌和小肠结肠炎耶尔森菌O9，一种事实混淆了抗O157抗体检测方法的解释。为了解决这个问题，使用 E设计了一种封闭酶联免疫吸附测定（bELISA）。大肠杆菌O157：H7 LPS作为抗原和对 E特异的单克隆抗体。大肠杆菌O157（称为13B3）作为竞争抗体。 bELISA与间接ELISA（iELISA）具有同等的敏感性，并且比间接ELISA（iELISA）具有更高的特异性，可以检测实验接种O157：H7的牛血清中的抗O157抗体。来自天真小母牛的血清中只有13％接种了B疫苗或进行了实验性感染。流产的抗O157 bELISA滴度增加，而抗O157 iELISA滴度增加61％。 bELISA是检测因暴露于 E而产生的血清抗体的灵敏且特异的方法。大肠杆菌O157。

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