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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Decreased Superoxide Production, Degranulation, Tumor Necrosis Factor Alpha Secretion, and CD11b/CD18 Receptor Expression by Adherent Monocytes from Preterm Infants
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Decreased Superoxide Production, Degranulation, Tumor Necrosis Factor Alpha Secretion, and CD11b/CD18 Receptor Expression by Adherent Monocytes from Preterm Infants

机译：早产婴儿粘附单核细胞的超氧化物生成，去颗粒化，肿瘤坏死因子α分泌和CD11b / CD18受体表达降低

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Preterm infants have an increased incidence of infection, which is principally due to deficiencies in neonatal host defense mechanisms. Monocyte adherence is important in localizing cells at sites of infection and is associated with enhanced antimicrobial functions. We isolated cord blood monocytes from preterm and full-term infants to study their adhesion and immune functions, including superoxide (O2 ?) generation, degranulation, and cytokine secretion and their adhesion receptors. O2 ?production and degranulation were significantly diminished, by 28 and 37%, respectively, in adherent monocytes from preterm infants compared to full-term infants (P < 0.05); however, these differences were not seen in freshly isolated cells. We also observed a significant decrease of 35% in tumor necrosis factor alpha secretion by lipopolysaccharide-stimulated adherent monocytes from preterm infants compared to full-term infants (P < 0.05); however, this difference was not observed in interleukin-1β or interleukin-6 production by the monocytes. The cell surface expression of the CD11b/CD18 adhesion receptor subunits was significantly decreased (by 60 and 52%, respectively) in monocytes from preterm infants compared to full-term infants (P < 0.01). The cascade of the immune response to infection involves monocyte upregulation and adherence via CD11b/CD18 receptors followed by cell activation and the release of cytokines and bactericidal products. We speculate that monocyte adherence factors may be important in the modulation of immune responses in preterm infants.
机译：早产儿的感染发生率增加，这主要是由于新生儿宿主防御机制的缺陷。单核细胞的粘附对于将细胞定位在感染部位很重要，并且与增强的抗菌功能有关。我们从早产儿和足月儿中分离了脐带血单核细胞，以研究它们的黏附和免疫功能，包括超氧化物（O 2 ？）的产生，脱颗粒和细胞因子的分泌及其功能。粘附受体。与足月儿相比，早产儿的粘附单核细胞中O 2 ？的产生和脱颗粒分别显着减少了28％和37％（ P < / em> <0.05）;但是，这些差异在新鲜分离的细胞中看不到。我们还观察到，与足月儿相比，早产儿脂多糖刺激的贴壁单核细胞分泌的肿瘤坏死因子α减少了35％（ P <0.05）;然而，在单核细胞的白细胞介素1β或白细胞介素6的产生中未观察到这种差异。与足月儿相比，早产儿单核细胞中CD11b / CD18粘附受体亚基的细胞表面表达显着降低（分别降低了60％和52％）（ P <0.01）。对感染的免疫反应的级联反应涉及单核细胞上调和通过CD11b / CD18受体的粘附，随后是细胞活化以及细胞因子和杀菌产物的释放。我们推测单核细胞粘附因子可能在早产儿免疫反应的调节中很重要。

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