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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Validation of the Indirect MAP1-B Enzyme-Linked Immunosorbent Assay for Diagnosis of Experimental Cowdria ruminantium Infection in Small Ruminants
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Validation of the Indirect MAP1-B Enzyme-Linked Immunosorbent Assay for Diagnosis of Experimental Cowdria ruminantium Infection in Small Ruminants

机译：间接MAP1-B酶联免疫吸附法对小反刍动物实验性反刍动物反刍动物感染诊断的验证

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The major antigenic protein 1 fragment B (MAP1-B) enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Cowdria ruminantium infections was validated to determine cutoff values and evaluate its diagnostic performance with sheep and goat sera.Cowdria-infected populations consisted of 48 sheep and 44 goats, while the noninfected populations consisted of 64 sheep and 107 goats. Cutoff values were determined by two-graph receiver-operating characteristic (TG-ROC) curves. The cutoff value was set at 31 and 26.6% of the positive control reference samples for sheep and goat sera, respectively. The test’s diagnostic performance was evaluated with measurements of the area under the concentration-time curve (AUC) of the ROC curves and by the valid range proportion (VRP). The AUCs were 0.978 for sheep sera and 0.989 for goat sera. The VRP for both sheep and goat sera was approximately 1.0. The intermediate range (IR), which defines results that are neither positive nor negative, was 0 for goat sera and 2.81 for sheep sera. In an ideal test, the AUC and VRP would be 1.0 and the IR would be 0. In this study these parameters were close to those of an ideal test. It is concluded that the MAP1-B ELISA is a useful test for the diagnosis of C. ruminantiuminfection in small ruminants.
机译：验证了主要的抗原蛋白1片段B（MAP1-B）酶联免疫吸附测定（ELISA）诊断 Cowdria ruminantium 感染的效果，以确定临界值并评估其在绵羊和山羊血清中的诊断性能。 Cowdria 感染的种群包括48只绵羊和44只山羊，而未感染的种群包括64只绵羊和107只山羊。截止值由两幅图的接收器工作特性曲线（TG-ROC）确定。绵羊和山羊血清的临界值分别设定为阳性对照参考样品的31.6％和26.6％。通过测量ROC曲线的浓度时间曲线（AUC）下的面积和有效范围比例（VRP）来评估测试的诊断性能。绵羊血清的AUC为0.978，山羊血清的AUC为0.989。绵羊和山羊血清的VRP均约为1.0。中间范围（IR）定义的结果既不是阳性也不是阴性，山羊血清为0，绵羊血清为2.81。在理想测试中，AUC和VRP为1.0，IR为0。在这项研究中，这些参数接近理想测试的参数。结论是，MAP1-B ELISA是诊断 C的有用方法。小反刍动物的反刍动物感染。

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