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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Helicobacter pylori Heat Shock Protein A: Serologic Responses and Genetic Diversity
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Helicobacter pylori Heat Shock Protein A: Serologic Responses and Genetic Diversity

机译：幽门螺杆菌热激蛋白A：血清学反应和遗传多样性。

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Helicobacter pylori synthesizes an unusual GroES homolog, heat shock protein A (HspA). The present study was aimed at an assessment of the serological response to HspA in a group of Chinese patients with defined gastroduodenal pathologies and determination of whether diversity is present in the nucleotide sequences encoding HspA in isolates from these patients. Serum samples collected from 154 patients who had an upper gastrointestinal pathology and the presence of H. pylori defined by biopsy were tested for an immunoglobulin G (IgG) serologic response to H. pylori HspA by an enzyme linked immunosorbant assay. HspA-encoding nucleotide sequences in H. pylori isolates from 14 patients (7 seropositive and 7 seronegative for HspA) were analyzed by PCR and direct sequencing of the PCR products. The sequencing results were compared to those of 48 isolates from other parts of the world. Of the 154 known H. pylori-positive patients, 54 (35.1%) were seropositive for HspA. The A domain (GroES homology) of HspA was highly conserved in the 14 isolates tested. Although the B domain (metal-binding site unique to H. pylori) resembled that in the known major variant, particular amino acid substitutions allowed definition of an HspA variant associated with isolates from East Asia. There were no associations between patient characteristics and HspA seropositivity or amino acid sequences. We confirmed in this study that the clinical outcomes of H. pylori infection are not related to HspA antigenicity or to sequence variation. However, B-domain sequence variation may be a marker for the study of the genetic diversity of H. pylori strains of different geographic origins.
机译：幽门螺杆菌（Helicobacter pylori）合成了一种异常的GroES同源物，即热激蛋白A（HspA）。本研究旨在评估一组具有明确胃十二指肠病理学的中国患者对HspA的血清学反应，并确定这些患者分离株中编码HspA的核苷酸序列中是否存在多样性。从154名具有上消化道病理和 H的患者中收集血清。通过活检确定的幽门螺旋杆菌对 H的免疫球蛋白G（IgG）血清学反应进行了测试。酶联免疫吸附法测定幽门螺杆菌HspA。 H中编码HspA的核苷酸序列。通过PCR和PCR产物的直接测序分析了14例患者的幽门螺杆菌分离株（HspA的7份血清阳性和7份血清阴性）。将测序结果与来自世界其他地区的48个分离株进行了比较。在154个已知的H中。幽门螺杆菌阳性的患者中，有54名（35.1％）的HspA血清阳性。 HspA的A结构域（GroES同源性）在14个分离株中高度保守。尽管B结构域（幽门螺杆菌特有的金属结合位点）与已知的主要变体相似，但特定的氨基酸取代允许定义与来自东亚的分离物有关的HspA变体。患者特征与HspA血清阳性或氨基酸序列之间没有关联。我们在这项研究中证实了 H的临床结局。幽门螺杆菌感染与HspA抗原性或序列变异无关。然而，B结构域序列变异可能是研究 H遗传多样性的标志。不同地理来源的幽门螺杆菌菌株

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