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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Deficient Antibody-Dependent Cellular Cytotoxicity against Human Immunodeficiency Virus (HIV)-Expressing Target Cells in Perinatal HIV Infection
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Deficient Antibody-Dependent Cellular Cytotoxicity against Human Immunodeficiency Virus (HIV)-Expressing Target Cells in Perinatal HIV Infection

机译：围产期HIV感染对人类免疫缺陷病毒（HIV）表达靶细胞的抗体依赖性细胞毒性不足。

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Peripheral blood mononuclear cells (PBMC) of human immunodeficiency virus (HIV)-infected children, age-matched HIV-seronegative controls, and HIV-infected asymptomatic and symptomatic adults were compared for their ability to mediate antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) cell-mediated cytotoxicity against target cells expressing HIV or herpes simplex virus (HSV) antigens. Target cells consisted of CD4 lymphocytes purified from PBMC of HIV-seronegative adults and incubated with the IIIB strain of HIV, HUT78 cells chronically infected with IIIB, and HSV-infected human fibroblasts. PBMC of asymptomatic HIV-infected adults were generally able to lyse CD4 cells expressing HIV antigens. Direct correlation was found between the magnitude of lysis and absolute CD4 cell counts in these individuals. In contrast to these results, PBMC from HIV-infected children were generally unable to lyse IIIB-expressing CD4 cells, regardless of the children’s clinical status, age, or absolute CD4 cell counts. Cells from HIV-seronegative adults and children did not directly lyse these target cells either but, in contrast to cells of HIV-seropositive children, were able to mediate cell lysis when serum from an HIV-seropositive adult was added. However, effector cells from these HIV-infected children were able to mediate both ADCC against HSV-infected fibroblasts and NK cell-mediated cytotoxicity against IIIB-infected HUT78 cells. Reduced ability of PBMC from vertically HIV-infected children to mediate ADCC against HIV antigen-expressing CD4 cells may contribute to rapid progression to AIDS.
机译：比较了人类免疫缺陷病毒（HIV）感染的儿童，年龄匹配的HIV血清阴性对照以及HIV感染的无症状和有症状成年人的外周血单个核细胞（PBMC）介导抗体依赖性细胞毒性（ADCC）和天然杀伤（NK）细胞介导的针对表达HIV或单纯疱疹病毒（HSV）抗原的靶细胞的细胞毒性。靶细胞由从HIV血清阴性成年人的PBMC纯化并与HIV IIIB菌株，长期感染IIIB的HUT78细胞和HSV感染的人类成纤维细胞温育而成的CD4淋巴细胞组成。无症状HIV感染的成年人的PBMC通常能够裂解表达HIV抗原的CD4细胞。在这些个体的裂解量和绝对CD4细胞计数之间发现直接相关。与这些结果相反，来自HIV感染儿童的PBMC通常无法裂解表达IIIB的CD4细胞，无论儿童的临床状况，年龄或CD4细胞的绝对计数如何。来自HIV血清阳性成人和儿童的细胞也没有直接裂解这些靶细胞，但是与HIV血清阳性儿童的细胞相反，当添加来自HIV血清阳性成人的血清时，它们能够介导细胞裂解。但是，来自这些被HIV感染儿童的效应细胞能够介导ADCC抵抗HSV感染的成纤维细胞和NK细胞介导的针对IIIB感染的HUT78细胞的细胞毒性。垂直感染HIV的儿童的PBMC介导ADCC抵抗表达HIV抗原的CD4细胞的能力降低，可能有助于迅速发展为AIDS。

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