Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with a Borrelia burgdorferi Recombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks

Yung-Fu Chang; Sean P. McDonough; Chao-Fu Chang; Kwang-Soon Shin; William Yen; Thomas Divers

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Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with a Borrelia burgdorferi Recombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks

机译：接种疏螺旋体伯氏疏螺旋体重组OspA疫苗的小马中的人粒细胞埃希氏病菌感染，并受到暴露于自然感染的壁虱的挑战

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A pony was vaccinated with recombinant OspA vaccine (rOspA) and then exposed 3 months later to Borrelia burgdorferi-infected ticks (Ixodes scapularis) collected in Westchester County, N.Y. At 2 weeks after tick exposure, the pony developed a high fever (105°F). Buffy coat smears showed that 20% of neutrophils contained ehrlichial inclusion bodies (morulae). Flunixin Meglumine (1 g daily) was given for 2 days, and the body temperature returned to normal. PCR for ehrlichial DNA was performed on blood samples for 10 consecutive days beginning when the pony was first febrile. This pony was monitored for another 3.5 months but developed no further clinical signs. The 44-kDa immunodominant human granulocytic ehrlichiosis antigen gene was amplified by PCR and cloned into a pCR2.1 vector. DNA sequence analysis of this gene showed it was only 8 bp different (99% identity) from the results reported by others (J.W. Ijdo et al., Infect. Immun. 66:3264–3269, 1998). Western blot analysis, growth inhibition assays, and repeated attempts to isolate B. burgdorferi all demonstrated the pony was protected against B. burgdorferi infection. These results highlight the potential for ticks to harbor and transmit several pathogens simultaneously, which further complicates the diagnosis and vaccination of these emerging tick-borne diseases.

机译：小马接种重组OspA疫苗（rOspA），然后在3个月后暴露于纽约州威彻斯特县收集的 Borrelia burgdorferi 感染的tick（ sxulars scapularis ）。 tick暴露后，小马发高烧（105°F）。血沉棕黄的外套涂片显示，20％的中性粒细胞含有埃希氏包涵体（桑ula）。给予氟尼辛葡甲胺（每日1 g）2天，体温恢复正常。从小马首次发热开始，连续10天对血样进行PCR检测。对该小马进行了另外3.5个月的监测，但没有进一步的临床体征。通过PCR扩增44kDa免疫优势的人粒细胞性埃希氏菌病抗原基因，并将其克隆到pCR2.1载体中。该基因的DNA序列分析表明，它与其他人报道的结果仅相差8 bp（99％相同）（J.W。Ijdo等人，Infect。Immun。66：3264-3269，1998）。蛋白质印迹分析，生长抑制测定法和反复尝试分离B的尝试。 burgdorferi 都表明小马受到了 B的保护。 burgdorferi 感染。这些结果凸显了壁虱同时藏匿和传播几种病原体的潜力，这使这些新兴壁虱传播疾病的诊断和疫苗接种更加复杂。 展开▼

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《Clinical and diagnostic laboratory immunology》 |2000年第1期|共4页

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Yung-Fu Chang; Sean P. McDonough; Chao-Fu Chang; Kwang-Soon Shin; William Yen; Thomas Divers;
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中图分类诊断学;

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1. Molecular Evidence of Coinfection of Borrelia burgdorferi Sensu Lato, Human Granulocytic Ehrlichiosis Agent, and Babesia microti in Ticks From Northwestern Poland [J] . B. Skotarczak A. Rymaszewska B. Wodecka and M. Sawczuk Journal of Parasitology . 2003,第1期

机译：波兰西北部壁虱中勃氏疏螺旋体，人类粒细胞埃希氏病病原体和小Ba虫共感染的分子证据

2. Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in Northern Colorado. [J] . Zeidner NS, Burkot TR, Massung R, The Journal of Infectious Diseases . 2000,第2期

机译：Ixodes spinipalpis壁虱传播人类粒细胞性埃希氏病病原体：北科罗拉多州伯氏疏螺旋体双重感染的虫卵周期的证据。

3. Molecular Evidence of Coinfection of Ticks with Borrelia burgdorferi Sensu Lato and the Human Granulocytic Ehrlichiosis Agent in Switzerland [J] . Christian M. Leutenegger, Nicola Pusterla, Caroline N. Mislin, Journal of Clinical Microbiology . 1999,第10期

机译：瑞士伯氏疏螺旋体和人类粒细胞埃希氏病病菌共同感染Ti虫的分子证据

4. Borrelia burgdorferi Outer surface protein A (OspA): A tick transmission factor and a Lyme disease vaccine. [D] . Gipson, Clay Livingston. 2004

机译：伯氏疏螺旋体（Borrelia burgdorferi）外表面蛋白A（OspA）：tick传播因子和莱姆病疫苗。

5. Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with a Borrelia burgdorferi Recombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks [O] . Yung-Fu Chang, Sean P. McDonough, Chao-Fu Chang, 2000

机译：接种疏螺旋体伯氏疏螺旋体重组OspA疫苗的小马中的人粒细胞埃希氏病菌感染并受到暴露于自然感染的壁虱的挑战

6. Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with a Borrelia burgdorferi Recombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks [O] . Chang, Yung-Fu, McDonough, Sean P., Chang, Chao-Fu, 2000

机译：在接种了疏螺旋体伯氏疏螺旋体重组OspA疫苗的小马中，人类小粒性囊虫病病原感染并受到暴露于自然感染的壁虱的挑战

1. Isolated dna molecule isolated protein or polypeptide process to vaccinate mammals against mycobacterium tuberculosis infection recombinant dna expression system vaccine host to prevent infection and mammalian disease by mycobacterium tuberculosis process to vaccinate mammals isolated antibody or binding moiety same process and composition to passively imminate mammals infected with mycobacterium tuberculosis processes to detect mycobacterium tuber [P] . 外国专利： BR9607406A . 1998-07-07

机译：分离的dna分子分离的蛋白质或多肽过程，用于对哺乳动物进行结核分枝杆菌感染的疫苗接种重组dna表达系统疫苗宿主，用于预防分枝杆菌的感染和哺乳动物疾病，以对哺乳动物分离的抗体或结合部分进行疫苗接种，相同的过程和组成，以被动地模仿被分枝杆菌感染的哺乳动物检测结核分枝杆菌的结核病过程

2. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . 外国专利： BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。

3. RECOMBINANT PROTEINS FROM BORRELIA, ITS PRODUCTION PROCESS, VACCINE AGAINST BORRELIA INFECTION AND IMMUNODIAGNOSTIC AGENT FOR ITS DETECTION [P] . 外国专利： PT100980B . 1999-07-30

机译：鲍氏杆菌的重组蛋白，其生产过程，抗鲍氏菌感染的疫苗和免疫诊断试剂

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Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with a Borrelia burgdorferi Recombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks

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