Sensitive and Specific Identification of Neospora caninum Infection of Cattle Based on Detection of Serum Antibodies to Recombinant Ncp29

Daniel K. Howe; Keliang Tang; Patricia A. Conrad; Karen Sverlow; J. P. Dubey; L. David Sibley

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Sensitive and Specific Identification of Neospora caninum Infection of Cattle Based on Detection of Serum Antibodies to Recombinant Ncp29

机译：基于重组Ncp29血清抗体检测的牛新孢子虫感染的敏感性和特异性鉴定

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Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between N. caninum and closely related parasites Toxoplasma gondii and Sarcocystis cruzi. To provide a sensitive and specific assay for detecting antibodies to N. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known as Ncp29 (rNcp29), which is a major surface protein of the parasite. Ncp29 is encoded by a gene that is homologous to the SAG1 gene previously characterized from T. gondii. An enzyme-linked immunosorbent assay (ELISA) was used to screen animals for the presence of serum antibodies specific to rNcp29. The rNcp29 ELISA readily distinguished between cattle known to be infected with N. caninum (optical density [OD] > 1.2 at 1:500 or greater dilution) and negative controls (OD < 0.5 at 1:500). Additionally, sera from animals that were infected with T. gondii or S. cruzi were negative. The rNcp29 ELISA developed here provides a specific and sensitive assay for detecting neosporosis in cattle.

机译：新孢子虫病是由原生动物犬新孢子虫引起的一种经济上重要的奶牛疾病。新孢子虫病的诊断测试由于抗体与 N之间相似的抗原发生交叉反应的可能性而变得复杂。犬和与之密切相关的寄生虫弓形虫和 Sarcocystis cruzi 。为检测 N抗体提供一种灵敏而特异性的检测方法。在被感染动物的血清中，我们研究了一种重组形式的抗原Ncp29（rNcp29），它是该寄生虫的主要表面蛋白。 Ncp29由与先前由 T表征的 SAG1 基因同源的基因编码。用酶联免疫吸附试验（ELISA）筛选动物中是否存在rNcp29特异性血清抗体。 rNcp29 ELISA易于区分已知感染了Nem的牛。犬（稀释度为1：500或更高时，光密度[OD]> 1.2）和阴性对照（稀释度为1：500时，OD <0.5）。此外，还感染了 T的动物的血清。 gondii 或 S。 Cruzi 是负面的。此处开发的rNcp29 ELISA提供了一种特异性和灵敏的检测牛新孢子虫病的方法。 展开▼

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《Clinical and diagnostic laboratory immunology》 |2002年第3期|共5页

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Daniel K. Howe; Keliang Tang; Patricia A. Conrad; Karen Sverlow; J. P. Dubey; L. David Sibley;
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中图分类诊断学;

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1. Validation of a Commercially Available Monoclonal Antibody-Based Competitive-Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to Neospora caninum in Cattle [J] . Timothy V. Baszler, Scott Adams, John Vander-Schalie, Journal of Clinical Microbiology . 2001,第11期

机译：基于市售单克隆抗体的竞争抑制酶联免疫吸附法检测牛新孢子虫血清抗体的验证

2. NEOSPORA CANINUM-SPECIFIC ANTIBODIES IN FREE-RANGING WHITE-LIPPED PECCARIES ( TAYASSU PECARI) FROM THE PERUVIAN AMAZON: DETECTION OF ANTIBODIES IN SERUM AND EVALUATION OF INDIRECT FLUORESCENT ANTIBODY TEST WITH HETEROLOGOUS SECONDARY ANTIBODY. [J] . Marcos Gomes Lopes, Monica Romero Solorio, Herbert Sousa Soares, Journal of Zoo and Wildlife Medicine . 2018,第3期

机译：来自秘鲁亚马逊的自由的白嘴巴（Tayassu Pecari）的新孢子岛癌症特异性抗体：检测血清中的抗体和与异源二抗的间接荧光抗体试验的评价。

3. Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle. [J] . Moraveji M., Hosseini A., Moghaddar N., Veterinary Parasitology . 2012,第2a4期

机译：用重组NcSAG1开发乳胶凝集试验，用于快速检测牛新孢子虫的抗体。

4. ESTIMATED ECONOMIC LOSSES DUE TO NEOSPORA CANINUM INFECTION IN DAIRY HERDS WITH AND WITHOUT A HISTORY OF NEOSPORA CANINUM ASSOCIATED ABORTION EPIDEMICS [C] . C.J.M. BARTELS, H. HOGEVEEN, G. VAN SCHAIK, Meeting of the Society for Veterinary Epidemiology and Preventive Medicine . 2006

机译：由于Neospora Caninum相关堕胎流行病史的乳制品牛群中Neospora Caninum感染导致的经济损失估计

5. Associations Among Beef Cattle Genotypes, Neospora caninum Infections, and Reproductive Performance [D] . ?Page, Ryan James 2020

机译：肉牛基因型中的关联，Neospora caninum感染和生殖性能

6. Sensitive and Specific Identification of Neospora caninum Infection of Cattle Based on Detection of Serum Antibodies to Recombinant Ncp29 [O] . Daniel K. Howe, Keliang Tang, Patricia A. Conrad, 2002

机译：基于重组Ncp29血清抗体检测的牛新孢子虫感染的敏感性和特异性鉴定

7. Sensitive and Specific Identification of Neospora caninum Infection of Cattle Based on Detection of Serum Antibodies to Recombinant Ncp29 [O] . Howe, Daniel K., Tang, Keliang, Conrad, Patricia A., 2002

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4. Hydrophilic eimeria polypeptide, DNA fragment, recombinant DNA molecule, live recombinant carrier, host cell, vaccine capable of protecting poultry against infection by eimeria, antibody, and processes for the preparation of antibodies against a polypeptide, for the preparation of a vaccine to fight eimeria infections, to detect eimeria parasites in poultry, and to detect antibodies against eimeria parasites in poultry serum [P] . 外国专利： BR9904488A . 2001-01-23

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Sensitive and Specific Identification of Neospora caninum Infection of Cattle Based on Detection of Serum Antibodies to Recombinant Ncp29

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