Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

Alessandra Xavier Pardini; Regina Helena Peralta; Adelaide José Vaz; Luis dos Ramos Machado; José Mauro Peralta

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Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

机译：猪带en虫囊尾er抗原制剂在检测神经囊尾osis病患者脑脊液样品中的抗体的用途

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Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.

机译：from虫Ta囊泡液中的抗原提取物和凝集素伴刀豆球蛋白A经亲和层析纯化的级分和电泳分离的糖蛋白抗原均用于检测Ta虫Ta虫。 em> anticysticercus抗体。在酶联免疫吸附试验中，所有抗原的敏感性和特异性均为100％，重复性好。使用这三种抗原的免疫印迹，仅在患有神经囊尾osis病患者的脑脊液样本中鉴定了低分子量肽（18和14 kDa）。结果证实从 T纯化的抗原级分。狂犬病史氏杆菌是特定肽的重要来源，并被证明可有效检测抗 T。抗体。 展开▼

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《Clinical and diagnostic laboratory immunology》 |2002年第1期|共4页

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Alessandra Xavier Pardini; Regina Helena Peralta; Adelaide José Vaz; Luis dos Ramos Machado; José Mauro Peralta;
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中图分类诊断学;

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专利

1. Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens [J] . Adelaide José Vaz, Ednéia Casagranda Bueno, José Antonio Livramento, Arquivos de Neuro-Psiquiatria . 2000,第1期

机译：神经囊尾osis病：通过Ta虫ia虫和Ta虫Ta抗原的ELISA检测脑脊液，血清和唾液样品中的IgG，IgA和IgE抗体

2. The use of antigenic preparation of Taenia crassiceps cysticercus to detect antibodies in neurocysticercosis (Taenia Solium) [J] . Alessandra Xavier Pardini Arquivos de Neuro-Psiquiatria . 2000,第4期

机译：猪带en虫en虫抗原制剂的使用来检测神经囊虫病（Taenia Solium）中的抗体

3. Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples [J] . Elizabeth Maria Aparecida Barasnevicius Quagliato Revista da Sociedade Brasileira de Medicina Tropical . 2007,第2期

机译：使用ELISA对脑脊液样本评估Ta虫和and虫囊性囊性抗原对神经囊虫病的免疫诊断

4. A novel acoustofluidic device for antigen antibody interaction detection using electrochemical impedance measurement [C] . Tsai Tsung-Heng, Lee Chih-Kung International Conference on Electronic Packaging Technology . 2014

机译：一种使用电化学阻抗测量的用于抗原抗体相互作用检测的新型声流装置

5. Gamma-delta T lymphocytes: Immunoregulatory and effector functions during murine neurocysticercosis (Taenia solium). [D] . Cardona Bedoya, Astrid Elena. 2002

机译：γ-δT淋巴细胞：鼠神经囊尾rc病（Taenia solium）期间的免疫调节和效应子功能。

6. Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium) [O] . Alessandra Xavier Pardini, Regina Helena Peralta, Adelaide José Vaz, 2002

机译：猪带en虫囊尾er抗原制剂在检测神经囊尾osis病患者脑脊液样品中的抗体的用途

7. Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium) [O] . Pardini, Alessandra Xavier, Peralta, Regina Helena, Vaz, Adelaide José, 2002

机译：猪带en虫囊尾er抗原制剂在检测神经囊尾osis病患者脑脊液样品中的抗体的用途

8. Comparison of Immunoassays for Detection of Bacterial Antigens in Cerebrospinal Fluid from Meningitis Patients [R] . Sippel, J. E., Prato, C. M., Sugasawara, R. J. 1986

机译：脑膜炎患者脑脊液中细菌抗原检测的免疫分析比较

1. ELISA检测脑囊虫病患者脑脊液中囊尾蚴抗原 [J] . 张洪花 ,尹舜 . 中国人兽共患病杂志 . 1992,第001期

2. 脑出血患者的脑组织及脑脊液中弓形虫循环抗原及抗体检测 [J] . 韩辉 ,宋涛 ,孙丹 . 中风与神经疾病杂志 . 2007,第001期

1. THE RECOMBINANT CHIMERIC PROTEIN FOR THE DIAGNOSIS OF TAENIA SOLIUM INFECTION AND DIAGNOSTIC KITS COMPRISING THE SAME TOWARD A STANDARDIZED SERODIAGNOSIS OF TAENIA SOLIUM NEUROCYSTICERCOSIS [P] . 外国专利： WO2009157602A1 . 2009-12-30

机译：重组嵌合蛋白用于诊断TA虫病和诊断试剂盒，包括对TO虫病的标准血清学诊断

2. Kit or device used in the detection of Taenia solium and process employed in the detection of Taenia solium [P] . 外国专利： BRPI0506278A . 2007-10-02

机译：用于检测Ta虫的试剂盒或装置以及用于检测process虫的方法

3. methods and compositions for the detection of taenia solium larvae using a cloned antigen [P] . 外国专利： AT381713T . 2008-01-15

机译：克隆抗原检测虫幼虫的方法和组合物

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Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

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