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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Human humoral responses to antigens of Mycobacterium tuberculosis: immunodominance of high-molecular-mass antigens.
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Human humoral responses to antigens of Mycobacterium tuberculosis: immunodominance of high-molecular-mass antigens.

机译：人类对结核分枝杆菌抗原的体液反应：高分子质量抗原的免疫优势。

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The selection of antigens of Mycobacterium tuberculosis for most studies of humoral responses in tuberculosis patients has been restricted to molecules that were either immunodominant in immunized animals or amenable to biochemical purification rather than those that were reactive with the human immune system. Delineation of antigens that elicit humoral responses during the natural course of disease progression in humans has been hindered by the presence of cross-reactive antibodies to conserved regions on ubiquitous prokaryotic antigens in sera from healthy individuals and tuberculosis patients. The levels of cross-reactive antibodies in the sera were reduced by preadsorption with Escherichia coli lysates, prior to studying their reactivity against a large panel of M. tuberculosis antigens to which the human immune system may be exposed during natural infection and disease. Thus, reactivity against pools of secreted, cellular, and cell wall-associated antigens of M. tuberculosis was assessed by an enzyme-linked immunosorbent assay (ELISA). Initial results suggested that the secreted protein preparation contained antigens most frequently recognized by the humoral responses of pulmonary tuberculosis patients. The culture filtrate proteins were subsequently size fractionated by preparative polyacrylamide gel electrophoresis, characterized by reaction with murine monoclonal antibodies to known antigens of M. tuberculosis by an ELISA, and assessed for reactivity with tuberculous and nontuberculous sera. Results show that a secreted antigen of 88 kDa elicits a strong antibody response in a high percentage of patients with pulmonary tuberculosis. This and other antigens identified on the basis of their reactivity with patient sera may prove useful for developing serodiagnosis for tuberculosis.
机译：对于大多数结核病患者的体液反应研究，结核分枝杆菌抗原的选择仅限于在免疫动物中具有免疫优势或可进行生化纯化的分子，而不是与人类免疫系统具有反应性的分子。在人类疾病发展的自然过程中引发体液反应的抗原的描述已被针对健康个体和结核病患者血清中遍在原核抗原上保守区域的交叉反应抗体的存在所阻碍。在研究其与大范围的结核分枝杆菌抗原的反应性之前，先用大肠埃希氏菌裂解物预先吸附可降低血清中的交叉反应抗体的水平，人类免疫系统在自然感染和疾病期间可能会暴露于这些抗原。因此，通过酶联免疫吸附测定（ELISA）来评估针对结核分枝杆菌的分泌的，细胞的和细胞壁相关的抗原库的反应性。初步结果表明，分泌的蛋白质制剂含有最常被肺结核患者的体液反应识别的抗原。随后通过制备型聚丙烯酰胺凝胶电泳对培养滤液蛋白进行大小分级，其特征在于通过ELISA与鼠抗结核分枝杆菌已知抗原的单克隆抗体反应，并评估其与结核和非结核血清的反应性。结果表明，在高百分比的肺结核患者中，分泌的88 kDa抗原引起强烈的抗体反应。根据其与患者血清的反应性鉴定出的这种抗原和其他抗原可能被证明可用于发展结核病的血清诊断。

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