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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development of an enzyme-linked immunosorbent assay for measurement of serum-associated ALX40-4C.
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Development of an enzyme-linked immunosorbent assay for measurement of serum-associated ALX40-4C.

机译：酶联免疫吸附测定法的开发，用于测定血清相关的ALX40-4C。

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ALX40-4C is an antiretrovirus agent that has been found to have some inhibitory properties against human immunodeficiency virus (HIV) replication in vitro. The compound was designed as a competitor of the HIV Tat protein for TAR binding. In addition to its anti-HIV properties, it has demonstrated the ability to inhibit in vitro replication of herpes simplex virus types 1 and 2 as well as human cytomegalovirus. Subsequently, in vivo pharmacokinetic evaluation of ALX40-4C necessitated the establishment of a detection system for the measurement of ALX40-4C in subject serum. For this purpose, an indirect-competition enzyme-linked immunosorbent assay with generated rabbit anti-ALX40-4C antiserum was developed. The original assay took 12 h to complete and required many manipulations. Herein, we describe alterations to the system that resulted in the overall reduction in assay time and manipulation. We demonstrate that our alterations do not affect the specificity or sensitivity of the assay compared to that of the original system. ALX40-4C levels in spiked serum samples as well as drug levels from patient samples were used to validate the assay.
机译：ALX40-4C是一种抗逆转录病毒药物，已发现在体外对人免疫缺陷病毒（HIV）复制具有某些抑制作用。该化合物被设计为HIV Tat蛋白与TAR结合的竞争者。除了具有抗HIV特性外，它还具有抑制1型和2型单纯疱疹病毒以及人类巨细胞病毒体外复制的能力。随后，ALX40-4C的体内药代动力学评估需要建立用于检测受试者血清中ALX40-4C的检测系统。为此，开发了一种使用产生的兔抗ALX40-4C抗血清的间接竞争酶联免疫吸附测定法。原始测定需要12小时才能完成，并且需要许多操作。在本文中，我们描述了系统的变更，该变更导致测定时间和操作的总体减少。我们证明，与原始系统相比，我们的更改不会影响测定的特异性或敏感性。加标血清样品中的ALX40-4C水平以及患者样品中的药物水平用于验证测定。

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