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首页> 外文期刊>Comparative Cytogenetics >Cytogenetic analysis of Scinax auratus and Scinax eurydice (Anura, Hylidae) with emphasis on cytotaxonomy
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Cytogenetic analysis of Scinax auratus and Scinax eurydice (Anura, Hylidae) with emphasis on cytotaxonomy

机译:中华金丝猴和欧洲金丝猴(Anura,Hylidae)的细胞遗传学分析,重点是细胞分类学

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摘要

Scinax Wagler, 1830 is a species-rich genus of amphibians with relatively few detailed chromosomal reports. In this work, cytogenetic analyses of Scinax auratus (Wied-Neuwied, 1821) and Scinax eurydice (Bokermann, 1968) were carried out based on conventional (Giemsa staining, Ag-NOR and C-banding) and cytomolecular (base-specific fluorochrome staining and fluorescence in situ hybridization – FISH of ribosomal probes) techniques. Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinax ruber clade. Interpopulation chromosomal variation was observed in Scinax eurydice, indicating the occurrence of cryptic species. The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.
机译:Scinax Wagler,1830年,是两栖动物的一个物种丰富的属,很少有详细的染色体报道。在这项工作中,基于传统的(吉姆萨染色,Ag-NOR和C谱带)和细胞分子(碱基特异性荧光染料)对金枪鱼(Wied-Neuwied,1821)和金枪鱼(Bokermann,1968)进行了细胞遗传学分析。和荧光原位杂交–核糖体探针的FISH)技术。正如Scinax ruber进化枝中大多数物种所报道的,这两个物种具有相同的核型,在第11对上的活性核仁组织者区域和富含GC的异染色质。在Scinax eurydice中观察到种群间的染色体变异,表明存在隐性物种。首次报道了通过FISH对18S核糖体基因的定位。

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