• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Combined Cell Culture Enzyme-Linked Immunosorbent Assay for Quantification of Poliovirus Neutralization- Relevant Antibodies
【24h】

Combined Cell Culture Enzyme-Linked Immunosorbent Assay for Quantification of Poliovirus Neutralization- Relevant Antibodies

机译：结合细胞培养酶联免疫吸附试验定量脊髓灰质炎病毒中和相关抗体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A combined cell culture enzyme-linked immunosorbent assay (CCC-ELISA) was developed for measuring the neutralizing antipoliovirus antibodies in human sera. The binding of different concentrations of each of the three poliovirus types to BGM cells in the presence and absence of a constant dilution from each test and reference serum was measured in the CCC-ELISA. The titers of the viruses neutralized by each serum were measured with the titration curves and used for interpretation of neutralizing titers to the three poliovirus types. Analysis of human sera revealed that the sensitivity and specificity of the CCC-ELISA and the microneutralization assay were comparable. The CCC-ELISA is nonsubjective, rapid, and highly reproducible. Furthermore, the CCC-ELISA could potentially be used as a seroepidemiologic tool for assessment of the humoral response to the cell culture infectious viruses.
机译：开发了一种组合的细胞培养酶联免疫吸附试验（CCC-ELISA），用于测量人血清中的中和性抗脊髓灰质炎病毒抗体。在CCC-ELISA中测量在存在和不存在来自每种测试和参考血清的恒定稀释度的情况下，三种脊髓灰质炎病毒类型中的每种的不同浓度与BGM细胞的结合。用滴定曲线测量被每种血清中和的病毒的效价，并用于解释对三种脊髓灰质炎病毒的中和效价。对人血清的分析表明，CCC-ELISA和微中和测定的灵敏度和特异性是可比的。 CCC-ELISA是非主观的，快速且高度可重复的。此外，CCC-ELISA可以潜在地用作血清流行病学工具，以评估对细胞培养传染性病毒的体液反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号