• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antigenic Properties and Diagnostic Potential of Baculovirus-Expressed Infectious Bursal Disease Virus Proteins VPX and VP3
【24h】

Antigenic Properties and Diagnostic Potential of Baculovirus-Expressed Infectious Bursal Disease Virus Proteins VPX and VP3

机译：杆状病毒表达的传染性法氏囊病病毒蛋白VPX和VP3的抗原性和诊断潜力

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The routine technique for detecting antibodies specific to infectious bursal disease virus (IBDV) is a serological evaluation by enzyme-linked immunosorbent assay (ELISA) with preparations of whole virions as the antigens. To avoid using complete virus in the standard technique, we have developed two new antigens through the expression of the VPX and VP3 genes in insect cells. VPX and especially VP3 were expressed at high levels in insect cells and simple to purify. The immunogenicity of both proteins was similar to that of the native virus. VPX was able to elicit neutralizing antibodies but VP3 was not. Purified VPX and VP3 were tested in an indirect ELISA with more than 300 chicken sera. There was an excellent correlation between the results of the ELISA using VPX and those of the two commercial kits. VP3 did not perform as well as VPX, and the linear correlation was significantly lower. A comparison with the standard reference technique, seroneutralization, showed that the indirect ELISA was more sensitive. Therefore, VPX-based ELISA is a good alternative to conventional ELISAs that use whole virions.
机译：用于检测对传染性法氏囊病病毒（IBDV）特异的抗体的常规技术是通过酶联免疫吸附测定（ELISA）进行血清学评估，并以完整的病毒体制剂作为抗原。为了避免在标准技术中使用完整病毒，我们通过在昆虫细胞中表达VPX和VP3基因开发了两种新抗原。 VPX，尤其是VP3在昆虫细胞中高水平表达且易于纯化。两种蛋白质的免疫原性与天然病毒相似。 VPX能够引发中和抗体，但VP3不能。纯化的VPX和VP3在间接ELISA中用300多种鸡血清进行了测试。使用VPX的ELISA结果与两种商业试剂盒的结果之间存在极好的相关性。 VP3的性能不如VPX，并且线性相关性明显较低。与标准参考技术Serututralization的比较表明，间接ELISA更为灵敏。因此，基于VPX的ELISA是使用整个病毒体的常规ELISA的良好替代品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号