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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Earlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme Immunoassays
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Earlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme Immunoassays

机译：通过免疫复合物转移酶免疫测定法早期检测血清转化血清组中的人类免疫缺陷病毒1型p24抗原和针对p17抗原的免疫球蛋白G和M抗体

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For earlier diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, the sensitivities of immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody immunoglobulin G (IgG) to HIV-1 p17 antigen were improved approximately 25- and 90-fold, respectively, over those of the previous immunoassays by performing solid-phase immunoreactions with shaking and increasing the serum sample volumes, and immune complex transfer enzyme immunoassay of antibody IgM to p17 antigen was also performed in the same way as the improved immunoassay of antibody IgG to p17 antigen. By the improved immunoassays, p24 antigen and antibody IgG to p17 antigen were detected earlier in 32 and 53%, respectively, of the HIV-1 seroconversion serum panels tested than before the improvements, and p24 antigen was detected as early as or earlier than HIV-1 RNA by reverse transcriptase-PCR (RT-PCR) in all of the panels tested. In 4 panels out of 19 tested, antibody IgG to p17 antigen or both antibodies IgG and IgM to p17 antigen were detected earlier than p24 antigen and RNA, although the antibody levels declined slightly before their steep increases usually observed after p24 antigen and RNA. Thus, the window period in diagnosis of HIV-1 infection can be shortened by detection of p24 antigen with the improved immunoassay as much as by detection of RNA with RT-PCR and, in some cases, more by detection of antibodies IgG and IgM to p17 antigen with the improved immunoassays than by detections of p24 antigen with the improved immunoassay and RNA with RT-PCR.
机译：为了更早地诊断人类1型免疫缺陷病毒（HIV-1）感染，针对HIV-1 p24抗原和抗体免疫球蛋白G（IgG）对HIV-1 p17抗原的免疫复合物转移酶免疫测定的敏感性分别提高了约25和90通过摇动并增加血清样品体积进行固相免疫反应，分别比以前的免疫测定高两倍，并且还以与改良的免疫测定相同的方式进行了抗体IgM对p17抗原的免疫复合物转移酶免疫测定。 p17抗原的IgG抗体。通过改进的免疫测定，与改进之前相比，分别在32％和53％的HIV-1血清转化血清组中更早地检测到p24抗原和针对p17抗原的IgG，并且最早或早于HIV检测到p24抗原。通过逆转录酶-PCR（RT-PCR）在所有测试组中得到-1 RNA。在测试的19个样本中有4个样本中，p17抗原的IgG抗体或p17抗原的IgG和IgM抗体都早于p24抗原和RNA，尽管通常在p24抗原和RNA之后通常会急剧上升，但抗体水平却略有下降。因此，通过改进的免疫测定方法检测p24抗原，与通过RT-PCR检测RNA一样，可以缩短诊断HIV-1感染的窗口期，在某些情况下，还可以通过检测抗IgG抗体和IgM来缩短与通过改进的免疫测定法检测p24抗原和通过RT-PCR检测RNA相比，通过改进的免疫测定法检测到p17抗原。

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