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Evaluation of Antibodies against a Rubella Virus Neutralizing Domain for Determination of Immune Status

机译：评价风疹病毒中和域的抗体以确定免疫状态

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The protective immune responses against rubella virus (RV) are related to its neutralizing epitopes, an issue that is important to consider when assessing the immune status of patients with remote infection. In the present paper, we compare the antibodies detected by a synthetic-peptide-based enzyme immunoassay (EIA) with antibodies detected by the traditional technique of hemagglutination inhibition (HIA) in patients with remote RV infection. The synthetic peptide used as an antigen (SP15) represents a neutralizing epitope that corresponds to amino acids 208 to 239 of the E1 glycoprotein. The SP15-EIA was developed, all variables that affected the assay were standardized, and the test was validated using reference sera. Serum samples (n = 129) from patients with remote RV infection were tested by HIA and SP15-EIA. Discrepant sera were assayed by MEIA (IMX/Abbot). The comparison between HIA and SP15-EIA, taking HIA as the standard methodology for determining immune status, showed that SP15-EIA is very specific and sensitive for detecting protecting antibodies (specificity, 100%; sensitivity, 98.20%). This study demonstrates that antibodies against the neutralizing domain represented by SP15 would be important in the memory response after natural infection and may be a good tool in the determination of the true immune status of patients with remote infection with regard to RV.
机译：抗风疹病毒（RV）的保护性免疫应答与其中和表位有关，在评估远距离感染患者的免疫状况时必须考虑这一问题。在本文中，我们比较了基于合成肽的酶免疫测定（EIA）和远端RV感染患者的传统血凝抑制技术（HIA）检测到的抗体。用作抗原的合成肽（SP15）代表中和性表位，对应于E1糖蛋白的208至239位氨基酸。开发了SP15-EIA，所有影响测定的变量均已标准化，并使用参考血清验证了测试。通过HIA和SP15-EIA对远程RV感染患者的血清样本（ n = 129）进行了测试。通过MEIA（IMX / Abbot）测定差异血清。以HIA作为确定免疫状态的标准方法，对HIA和SP15-EIA进行比较，结果表明SP15-EIA对检测保护性抗体非常特异性和敏感性（特异性为100％;敏感性为98.20％）。这项研究表明，针对SP15代表的中和结构域的抗体在自然感染后的记忆反应中很重要，并且可能是确定远程感染患者关于RV的真实免疫状态的良好工具。

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