Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies

Sandra Romero-Steiner; Tamar Pilishvili; Jacquelyn S. Sampson; Scott E. Johnson; Annie Stinson; George M. Carlone

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Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies

机译：抗PsaA抗体抑制肺炎球菌对人鼻咽上皮细胞的粘附

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The role of pneumococcal (Pnc) surface adhesin A (PsaA) in the adherence of Streptococcus pneumoniae (pneumococcus) to host cells is not well defined. We examined the effect of anti-PsaA antibodies in an inhibition of adherence assay using Detroit 562 nasopharyngeal human epithelial cells. Rabbit polyclonal (Pab) anti-recombinant PsaA (rPsaA) sera, a purified mouse monoclonal antibody (MAb) (MAb 6F62G8E12), and 22 healthy adult sera with known anti-PsaA IgG levels (obtained by enzyme-linked immunosorbent assay) were evaluated for their abilities to inhibit Pnc adherence to confluent monolayers (measured as percent reduction in CFU counts compared to those of uninhibited controls). Pnc adherence was dependent on capsular phenotype (no or low adherence for opaque strains). With an inoculum of 104 to 105 bacteria/well, the mean ± standard deviation count in controls was 163 ± 32 CFU/well for transparent strains. Low adherence was observed for a PsaA-minus mutant even at higher inoculum doses. Mean percent inhibitions of adherence with Pab and MAb were 54 and 50%, respectively. Adult sera showed inhibition in a dose-response fashion with a range of 98 to 8%, depending on the serum anti-PsaA antibody concentration. Absorption of Pab with rPsaA restored Pnc adherence to control levels. Absorption of sera with a PsaA-minus mutant did not result in a significant decrease (P >0.05) of inhibition of adherence activity. Additionally, nearly 100% of Pnc adherence was inhibited by lipidated rPsaA at 2.5 μg/ml. Our data support the argument that PsaA is an adhesin that mediates Pnc adherence to human nasopharyngeal cells. This functional assay may be useful in evaluating antibodies elicited in response to PsaA vaccination.

机译：肺炎球菌（Pnc）表面粘附素A（PsaA）在肺炎链球菌（肺炎球菌）对宿主细胞的粘附中的作用尚不清楚。我们使用底特律562鼻咽人类上皮细胞检查了抗PsaA抗体在抑制粘附试验中的作用。评价了兔多克隆（Pab）抗重组PsaA（rPsaA）血清，纯化的小鼠单克隆抗体（MAb）（MAb 6F62G8E12）和22株具有已知抗PsaA IgG水平的健康成人血清（通过酶联免疫吸附试验获得）抑制Pnc粘附于汇合单层的能力（以与未抑制对照相比CFU计数减少的百分比来衡量）。 Pnc的依从性取决于荚膜的表型（不透明菌株的依从性或不依从性低）。当接种量为10 4 到10 5 /孔时，透明菌株的对照的平均±标准偏差计数为163±32 CFU /孔。即使在较高的接种剂量下，PsaA负突变型的依从性也很低。 Pab和MAb的平均抑制率分别为54％和50％。根据血清抗-PsaA抗体的浓度，成年血清以剂量反应的方式显示出98-8％的抑制作用。用rPsaA吸收Pab可将Pnc依从性恢复至对照水平。用PsaA-负突变体吸收血清不会导致粘附活性抑制作用显着降低（ P 展开▼

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《Clinical and diagnostic laboratory immunology》 |2003年第2期|共6页

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Sandra Romero-Steiner; Tamar Pilishvili; Jacquelyn S. Sampson; Scott E. Johnson; Annie Stinson; George M. Carlone;
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专利

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机译：重组肺炎球菌表面粘附素A（rPsaA）包被的颗粒对人鼻咽上皮细胞的粘附性，以评估抗PsaA功能抗体

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机译：人类对PhtD，PcpA和Ply的抗体可减少对人肺上皮细胞的粘附和肺炎链球菌对小鼠鼻咽的定植

3. PcpA of Streptococcus pneumoniae mediates adherence to nasopharyngeal and lung epithelial cells and elicits functional antibodies in humans [J] . KhanM.N., SharmaS.K., FilkinsL.M., Microbes and infection . 2012,第12期

机译：肺炎链球菌的PcpA介导对鼻咽和肺上皮细胞的粘附并在人类中引发功能性抗体

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机译：保护性McAb 1B5影响白色念珠菌对人上皮细胞和内皮细胞的粘附

5. A novel approach to control and regulate the classical complement pathway: Highly specific inhibition of C1q globular head binding to human IgG using an engineered single chain antibody variable fragment; recruiting C1q to HER2 /neu positive cells using truncated bispecific antibodies [D] . Hwang, Hee Young 2007

机译：一种控制和调节经典补体途径的新方法：使用工程化的单链抗体可变片段高度特异性地抑制C1q球头与人IgG的结合；使用截短的双特异性抗体将C1q募集到HER2 / neu阳性细胞

6. Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies [O] . Sandra Romero-Steiner, Tamar Pilishvili, Jacquelyn S. Sampson, 2003

机译：抗PsaA抗体抑制肺炎球菌对人鼻咽上皮细胞的粘附

7. Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies [O] . Romero-Steiner, Sandra, Pilishvili, Tamar, Sampson, Jacquelyn S., 2003

机译：抗PsaA抗体抑制肺炎球菌对人鼻咽上皮细胞的粘附

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机译：人源化抗体抗整合素α2，测定样品中是否含有整合素α2的方法，试剂盒，核酸分离，载体，宿主细胞，人源化抗体抗整合素α2的制备方法，筛选方法，组成，处理方法与整合素α2β1相关的疾病。抑制白细胞与胶原蛋白结合的方法，分子定位方法，使用抗整合素α2的人源化抗体，连接整合有抗整合素α2抗体的整合素α2的表位和表位

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Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies

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