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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Monoclonal Antibodies against Enterocytozoon bieneusi of Human Origin
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Monoclonal Antibodies against Enterocytozoon bieneusi of Human Origin

机译：人类起源的Enterocytozoon bieneusi单克隆抗体

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Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not cross-react with other human microsporidia or with other microorganisms normally present in stool. One of the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection in various human and mammalian populations and the environmental sources of infection.
机译：临床上， Enterocytozoon bieneusi 在感染微孢子虫的人类中最为重要，可导致人类免疫缺陷病毒/艾滋病患者的慢性腹泻，消瘦和胆管炎。缺乏免疫试剂的主要原因是缺乏用于Eem实验室繁殖的方法。比内西。我们最近描述了一种从感染的人的腹泻粪便中浓缩和纯化孢子的程序。纯化的孢子用于免疫小鼠，以产生和筛选针对 E的单克隆抗体（MAb）。比内西。产生并充分表征的八种免疫球蛋白M MAb与其他人类微孢子虫或正常存在于粪便中的其他微生物没有交叉反应。其中一种单克隆抗体2G4与 E反应。猴子和人粪便中的比涅西孢子孢子，没有背景荧光，使其成为理想的诊断试剂。它也可以识别寄生虫的细胞内阶段，适合于确定 E的组织分布。 bieneusi 在被感染的主机中。至少两种Eem的免疫优势抗原。分别存在33,000和35,000 Da的比尼西（bineusi），已被兔和小鼠抗血清识别。针对 E的单克隆抗体的可用性。 bieneusi 将大大简化人类对这种感染的诊断，并为流行病学调查提供工具，以了解各种人类和哺乳动物种群中感染的真实流行情况以及感染的环境来源。

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